Negative and non-negative numbers

slant and if negative it will be a darker red color. The LIA tube will also be used to test decarboxylate lysine, which will help us distinguish bacteria that will produce hydrogen sulfide from those who cannot. If positive for decarboxylate lysine it will have a purple butt, and if negative it will have a yellow butt. The Sulfide Indole Motility (SIM) blue cap tube is a semisolid agar and it is also used to determine sulfide production, indole formation and motility in Gram negative bacteria.…

Sporosarcina. A positive Acetion production is eliminated S. caseolyticus,S. gallinarum, S. hyicus subsp. hyicus, S. hyicus subsp. chromogenes, S. intermedius, S. lentus, S. saccharolyticus, S. sciuri, and S. simulans. A negative Oxidase result is eliminated M. kristinae and S. xylosus. A negative Urea hydrolysis is eliminated S. cohnii subsp.2, S. epidermidis, S. hominis, S. saprophyticus, S. warneri, and S. xylosus. And then, a positive Maltose result is eliminated S. capitis, S. carnosus, and…

My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown, we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture, therefore we are very confident that it is correctly identified. We identified that K.…

departments. This would allow one to determine which departments face the most risk and require the most changes. In the study conducted by Po-Liang et al. (2009), no significant differences in pathogenic contamination rates between ICU workstations and non-ICU workstations (7.4% vs. 5% respectively) was found. The findings were consistent to those reported by Engelhart et al. (2008) in that few differences were detected for the incidence of pathogens between room type; contamination rates for…

products (cheese , yoghurt etc..). In fact, they inhibit these microbes up to 5 orders of magnitude at refrigerator temperatures (6oC) without changing the quality of the food. The bacterial mixture is effective against some yeasts, molds, and gram-negative bacteria, but not other gram-positive bacteria. During the tests on the inhibition activities of Propionibacterium sp., cell-free supernatants containing the organic acid molecules that are products of fermentation were not as effective in…

2.0 INTRODUCTION Consumption of foods and beverages that do not have added preservative but able to have a longer shelf life has become very popular among the consumer. In order to improve the shelf life of a food product, adding natural food extracts that act as the antimicrobial are able to inhibit or supress the microbial growth (Martin et al 2009). Microbial growth, pH and the storage temperature are the important measure of the shelf life and quality of a food product (Fellers 1988;…

with accuracy the unknown bacteria. II. Identification Bacteria are divided into Gram Positive and Gram Negative based on the chemical composition of the cell wall. Gram stain, a differential stain, can be used to identify the shape, arrangement, and chemical composition of the microorganisms. In the chemical composition of the Gram stain, we can identify the two types of bacteria: Gram-Negative Bacteria which has a thin peptidoglycan, lipopolysaccharide, phospholipid, and lipoprotein cell…

INTRODUCTION There have been multiple staining techniques before the Gram stain came about in 1883. These old staining techniques brought about issues because they stained both the tissue and the cells so it was hard to differentiate between the two. Christian Gram is the inventor of the gram stain and found this technique when he was looking at Streptococcus pneumoniae in the lung tissue of people and animals (1). When working with these tissues, he found that this bacterium would take up a…

positive bacterium, one Gram-negative paracolon, and one Gram-negative coliform (Carson, 2016). The unknown sample assigned in this experiment was #19. After initially streaking the solution on clean streak plates and TSA agar, the identity was determined from a Gram-stain of a single, isolated colony; this was confirmed by isolation of that pure colony and viewing the pure sample in a Gram-stain under a microscope yielding a blue colored cocci morphology with no Gram-negative contamination.…

“See if you can guess what I am now … I’m a zit. Get it?” - Bluto Background: The purpose of the antiseptic lab was to show the effectiveness of multiple antiseptics: hydrogen Peroxide, Purell hand sanitizer, Water and Iodine. The procedure of the lab for the first day involved our group acquiring a cotton swab and using the swab to gather the Propionibacterium acnes (P. acnes) bacteria from various spots around our face, we then grabbed a Petri dish filled only with a thin layer of agar. We…