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    Tpk-Trypsin Lab Report

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    expect to see a reduction in the viral titer in the DF-1 cells that are stably expressing viperin compared to the DF-1 cells that are transfected with an empty vector. Objective 4: Identification of the critical regions and residues important for duck viperin function and localization. Viperin consists of three distinct domains; an N-terminal domain that varies considerably between species and contains both an amphipathic helix and a leucine zipper domain; the N-terminal domain plays an important role in binding to the cytosolic face of the ER, as well as its association with lipid droplets (Hinson & Cresswell 2009), the C-terminal domain of viperin is highly conserved, and its presence is critical for its antiviral function against the Flaviviridae members hepatitis C virus (HCV) and dengue virus (DENV) the center domain is also conservative between species, this domain has been shown to be important in viperin ability to limit both human immunodeficiency virus (HIV) (Nasr et al. 2012) and Bunyamwera virus (Carlton-Smith & Elliott 2012). In order to determine which of these three domains is essential to the viperin antiviral effect, Short and long N-terminal and C-terminal truncation mutants will be constructed, as well as mutations to SAM motifs in the central domain. These mutants will be used to evaluate the antiviral effect as well as the localization within the cells. I expect to see a major decline in the antiviral role with the mutations in the SAM motifs in the…

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    For my service project I did two types of volunteering. One was being the president of Kilmer's Student Council Association (SCA). In SCA, a group of seventh and eighth grade students, including myself, got together about every other week, for an hour, to discuss, plan, and promote student events. Some of these events included student dances, holiday/seasonal games at lunch, fun runs, fundraisers and Kilmer Spirit Week. First, we would discuss what materials and how much time we would need to…

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    Clendinnen recounts the history of the Yucatan peninsula once the Spanish arrived. She splits her recounting into two sections: the Spanish’s perceptive and the Mayan’s perspective. Clendinnen’s recounting the Spanish side of history demonstrates a struggle not only between the Spanish and the new land and its inhabitants, but also the internal conflicts between the Spanish settlers and the friars. At first she tells us how the Spaniards’ interactions with the natives consisted of tribute…

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    The protein that is in sequence one is Alpha-synuclein. Alpha-synuclein is a protein whose function in the healthy brain is currently unknown (Alpha-synuclein and Parkinson's Disease, n.d.). Parkinson's disease researchers take great interest in it because it is a major constituent of Lewy bodies, which are protein clumps that are the pathological hallmark of Parkinson’s disease (Alpha-synuclein and Parkinson's Disease, n.d.). Researchers work to characterize the protein's role in Parkinson’s…

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    Climate Change and Trade Networks as Causes of the Classic Maya Collapse The ancient Lowland Maya were a thriving and advanced society, capable of building great monuments able to survive to the present. It is therefore a mystery as to why, at the end of the Classic period from the eighth to eleventh century, Maya sites show signs of massive decline and desertion; this is referred to as the “collapse” of the Maya, though it was not an immediate or evenly distributed phenomenon (Douglas,…

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    Have you ever heard the saying, “wrong place, wrong time,” or maybe that life is about “being at the right place at the right time”? Or have you ever heard about the cartels and their crimes down in Mexico? Murders, kidnapping, drug trafficking, and lots of partying goes on every day by every cartel and by every drug lord south of the border. Unfortunately, these two topics have much in common in Mexico. Civilians that do not have anything to do with the cartels get caught up in these kind of…

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    Ehbp1 Research Paper

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    The full name of EHBP1 is "EH domain binding protein 1." The symbol for the gene is EHBP1. Other names for the EHBP1 gene are HPC12, NASCIN, and KIAA0903. EH Domain Binding Protein 1 is a protein coding gene. This gene codes for an Eps15 homology domain binding protein. Its protein type is a vesicle. The encoded protein may play a role in endocytic trafficking. It is associated with prostate cancer. This gene is commonly found within the cytosol and the plasma membrane of a cell. It's size is…

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    APOE Gene Research Paper

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    Sequence one’s protein was found to be apolipoprotein E. This protein is known as the APOE gene. The protein forms molecules called lipoproteins by combining fats in the body. Lipoproteins have the responsibility to package cholesterol and other fats, and send them through the bloodstream. To prevent disorders with this protein, it is important to maintain the right level of cholesterol, so that it does not affect the heart and blood vessels that could causes a heart attack, stroke, or a…

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    about the difficulties she went through from being a young middle schooler to attending college at Harvard. She tells various situations of having been raised in Washington, D.C. and later on moving to Hawaii. At the beginning of the article Lawrence is riding home from school on a bus when a white man boards it. He is the only white person on a bus full of melanin and yet he feels the need to say the n-word. The aftermath was unbearable for young Kimiko, she wrote, “As I walk home from the bus…

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    Moreover, while Bloom endorses the linearity of influence, and Borges highlights its fluidity, a third distinct perception emerges. Thompson (2014, p.114) utilises David Foster Wallace’s short story B.I. #59 as a framework from which to interrogate Bloom and Borges’ arguments, detecting, “throughout [Wallace’s] fiction, influence comes not only from the past (as in Bloom’s model), or from a future, anticipated text (as in Borges’s model) but also from the cultural present.” Consequently,…

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