Mutant

Pathogenic bacteria species have been resistant to antibiotics before humans even made antibiotics. These bacterial samples which have displayed natural antibiotic resistance are as such due to genetic variation and mutations. When a bacterial species is exposed to an antibiotic, there is a selective pressure working against the majority which is not resistant. This leads to a decrease in the population of non-resistant bacteria yet an increase in the population of resistant bacteria since they…

Additionally, this proposal expands on the serial passage approach to IAV host adaptation by pairing it with circular RNA sequencing technology, a novel approach to deep sequencing of RNAs that allows for the detection of extremely low frequency mutants by drastically reducing noise from PCR-induced mutations (Acevedo et al., 2014). Essentially, circularized RNA templates are used to generate concatameric DNAs which are used for library preparation and next generation sequencing. This…

happened, the scientists used a mutant fruit fly that was unable to make cuticular hydrocarbons. Because these flies do not make cuticular hydrocarbons, they do not care about who they mate with. Infact, these mutant fruit flies cannot tell the difference between male or female. The research scientists continued with their study by creating special scents and extracting cuticular hydrocarbon from some starch flies and some molasses flies, and then coating the mutant flies with hydrocarbons from…

GMOs (Genetically Modified Organism) are being used on almost everything that you eat. In the book Solitary By Alexander Gordon Smith the use GMOs to make workers for them turning the prisoners at The Furnace into mutants. Alex and his crew are trying to escape the Furnace. When they all become seized and are thrown in the hole. One of their other friends comes and releases them and they uncover more secrets about the prison as they try to escape again. In this journal I well be sharing three…

immunization may be on its way. The research article discusses the steps researchers took in order to find a cure in mice. They reported, “emrA1 mutant is safe and can be used at an intranasal immunization dose as high as 1x106 CFU without causing any adverse effects in immunized mice,” (M.M, C.S.B., 2015). The article goes on to report that the emrA1 mutant is cleared in the vaccinated mice by day 14 post-immunization. Through their research they found that the immunization did get rid of…

CFTR stands for cystic fibrosis transmembrane conductance regulator whose mutation commonly leads to the deletion of three nucleotides which code for the amino acid phenylalanine. The mutation, therefore, leads to the loss of this amino acid. The mutant CFTR leads to the disease as the incorrect protein folding is detected in the endoplasmic reticulum which leads the protein to be degraded and thus never reaches the cell membrane whereas with a normal CFTR protein, the protein actually reaches…

Murata-Hori used a negative mutant form of Aurora-B kinase, they noticed that the movement of chromosomes was abnormal (Murata-Hori & Wang, 2002). With the mutant form, the chromosomes was seen to be arranged into two elongated bundles on the sides of the spindle axis instead of forming a metaphase plate in the equatorial region of the cell (Murata-Hori & Wang, 2002). Since the mutant used was kinase-inactive, there were a number of other adverse effects seen. When this mutant was…

Introduction: One of the most common mass spectrometry–based proteomic methodologies used to day is the bottom up protein identification. First, the protein has to be digested into peptides using a protease. Then, the peptide will be submitted to the URMC Proteomics Resource and Laboratory for analysis using LC-MS/MS tandem mass spectrometry. In this approach, the peptides will be separated and introduced into a mass spectrometer using reversed phase liquid chromatography (RC-LC). This…

this engineered PSII antenna, the efficiency of light capture is increased. This experimental group will also be placed in nitrogen starved conditions and the photosynthetic (light) conditions will be identical to those in the control group. These mutant…

Every person has parents, but not everybody gets to grow up with them. Whether by death or just the lack of responsibility, many people must teach themselves as they have nobody to guide them. Parental guidance is exactly what children need though, otherwise they can’t tell right from wrong. In the story “Superassasin” by Lysley Tenorio, the main character makes decisions without any real thoughts behind them which comes from having no moral compasses in his life other than his comics. Because…