Monoclonal antibodies

technique to detect and quantify substances like proteins, antibodies, and hormones. The target antigen is immobilized to the bottom of the well and then an antigen-specific detection antibody is added. The detection antibody makes it possible to measure results. The three most common formats of ELISA would be direct assay, indirect assay, and sandwich assay. In direct assay, the antigen immobilizes to the bottom of the well where a labeled antibody attaches to it. In indirect assay, the the…

For over 55 years, humans have been flying in space. With an understanding of evolution, it has always been assumed that human bodies have adapted to the gravitational force found here on Earth. The space station and the shuttle that most NASA astronauts will stay in orbits 350 km above Earth, with a gravitational pull 90% stronger than it is on the ground (NASA Space Bones). Giancoli describes objects orbiting the Earth as feeling weightless because they are, in essence, free falling towards…

Numerous toxicants have some sought of an affect the human body. These come in a variety of ways, but in the process more often the most affected happens to be the respiratory, immune system, along with the liver. These are primary systems within the body that are vital in order for each of us function and survive. Therefore if by some chance these if any of these systems/organ manage to fail, the likely outcome would be that a person becomes very ill. While technology has come a long way, as…

Meditation is a viable treatment option for patients seeking to enhance their physical well-being. This paper will provide a theoretical framework on this topic before making its own argument. Everyone should seek to benefit their physical well-being because the majority of individuals are not as healthy as they would hope to be and our physical well-beings are of utmost importance. One approach that a multitude of individuals have utilized to enhance their physical well-being is meditation.…

A colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich Chemical Co.) assay was performed to measure the cell viability. Briefly, RAW 264.7 cells were treated with different concentrations of spermidine for 24 h or pretreated with spermidine for 1 h before stimulation with LPS for 24 h. After incubation, the medium was discarded, and MTT solution (5 mg/mL in phosphate-buffered saline, PBS) was added to each well and incubated for another 3 h at 37 °C. The…

From laboratory research and data, understanding is that the body’s immune system initiates an antibody response of IgM and IgG antibodies against the virus. Thus, serological or blood test and ELISA is use to diagnose the disease. These tests look for the presence of immunoglobulins. Reverse transcriptase PCR is use in labs for diagnosis because the virus is an alpha…

an immune response (1). Antibodies and antigens found in our immune system is done by immunoassays (2). The majority of immunological tests are based on the adaptive immune system. Substances recognized by our immune system are antigens. Injections are done by a trained health care professional to test for an immune response. The antibodies work together to fight antigens. Immunologists can inject antigens into the body and observe the reactions. Out of the five antibodies, IgG and IgE are most…

The KIR ligand incompatibility model, also known as the “ligand-ligand” model was first suggested by the Perugia group, and predicts NK alloreactivity in the GVHD direction when the recipient lacks expression of an iKIR ligand (figure 4a) 31-34. This seminal study demonstrated the first clinical evidence of the efficacy of adoptive immunotherapy via NK alloreactivity 31,32. Utilizing HLA-mismatched haploidentical donors, Ruggeri and colleagues evaluated ninety two patients with high risk…

Practical 2 Title: Du Test by Tube Method Aim: To perform the Du test by tube method Introduction: The D antigen is the most immunogenic antigen in the Rhesus blood group system. Rhesus positivity and negativity indicate the presence or absence of the D antigen on the surface of red blood cell. Du test is the further testing that is done to identify Weak D antigen when the cells being tested have failed to react with anti-D antisera at immediate spin. An indirect antiglobulin test is…

3. Why a malaria vaccine is required? When microorganism enter the body, the immune system fight these foreign antigens by production of T or B-lymphocytes that produces antibodies, which effectively neutralize foreign invaders. Human body take several days to produces antibodies, while microorganism attack and multiply in the body and produce serious illness before immune system can fit back, this is a reason behind the vaccine manufacturing and development (CDC, 20175; Sarkander et al., 2016;…