Molecular orbital

A. fischeriThe overall purpose of this study was to create a genomic library of Aliivibrio fischeri (A. fischeri) thus aiding in creating a restriction map of the lux operon. It also employs typical molecular techniques important for biologists to understand. In this portion of the lab, the chromosomal DNA (chDNA) will be isolated. Its purity will be measured using spectrophotometric analysis. Lastly, the DNA will be digested and verified via gel electrophoresis. A. fischeri is a gram…

Throughout our daily lives and even the stories we read, have people or at least something taking a risk. Do you think that it was worth it when you had taken the risk you did, or even the stories risks that they take? For some stories, the risks they take are also questionable and even heroic for others, even if it means there own lives on the line. We risk so many things in our daily lives, do i ask for the promotions, do I speak out on an opinion that people have, etc… I will be telling you…

Analysis In part one, the varied amounts of surface area and volume had an effect on the diffusion rates of the trials. The greater the ratio was between surface area to volume, the rate of diffusion would increase as well. For example, in cell three the surface area to volume ratio was 11.5 cm which resulted in a diffusion rate of .30 cm/min while cell one had a surface to volume ratio of 3 cm had a diffusion rate of .015 cm/min. This shows that when the surface area and volume are…

Purification and Quantitation of DNA and Plasmid Structure Labs 3 and 4 Biology 453 Clayton Pedigo September 14 and 21, 2016 Introduction: In this report, there are two labs being discussed. The first lab being lab 3 (purification and quantitation of plasmid DNA) involved lysing bacterial E.coli cells and retrieving the plasmid particles. The purpose of the second lab, lab 4 (plasmid DNA structure), was to use the concentrated DNA from lab 3 and compare…

to dissolve the formazan dye. The optical density was then read at 560 nm using a microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA) to determine the cell viability. Measurement of NO production…

GMO Labeling- The Argument against Labeling The labeling of Genetically Modified Organism is nearly as controversial as GMO’s themselves. Well-intentioned consumer groups have lobbied to have GMO products labeled to protect consumers. These groups argue that GMO’s may have the potential to be dangerous and should be labeled. Those that advise against labeling, industry, academics, and regulatory bodies sight the complexities and cost of labels that do not improve safety. Mankind has been…

perceive and transmit signals that cause the cell to react to changes. The set of all genes of an organism is called genotype. In all the cells of the same organism genotype is the same. Genetic engineering is the complex of technology which using molecular biological methods allow changing the structure of the genes and introducing foreign genes with the specified functions into the organism. However, the organism is transferred only…

Introduction In the United States a suspect in a crime is innocent until they are proven in court of law to be guilty beyond doubt(Cornell web), for now at least. This need to prove guilt in a suspect means the use of DNA to determine if the suspect was at least present at the crime scene is a powerful tool because no one person can change their DNA at will. The use of DNA as way to identify people has it 's origins in the United Kingdom because of a technique created by Dr. Alex Jeffreys used…

3.4 DISCUSSIONS 3.4.1 Phenotypic characterization Accurate description of cowpea genotypes is crucial towards making a decision to release a variety as well as conservation of germplasm. The identities of 38 cowpea genotypes were established by using morphological characteristics described as cowpea descriptors (Makanur et al., 2013). Both quantitative and qualitative parameters considered in the current work showed variations among the 38 cowpea genotypes. Variation in growth habit was evident…

4.1 Maintenance and sub-culturing of endophytic fungi The cultures were procured from already maintained repository in laboratory. The maintenance of the cultures involved preparation of Potato dextrose agar (PDA) plates, sub culturing of cultures and long term preservation. 4.1.1 Preparation of PDA (Potato dextrose agar) plates 39g of PDA was dissolved in 1000 ml double distilled water, stirred to mix properly and was transferred into 250 ml Erlenmeyer flasks and autoclaved at 121˚C at 15…