Molecular orbital

Exploring Bacterial Transformation Ian Nall BIO 181 | Section #73116 | Wednesday 1:30-4:15pm Abstract This experiment was designed to test the properties of bacterial transformation. The bacteria used was E. coli, the protein being added to it is called Green Fluorescent Protein or GFP from a jellyfish the Aequorea victoria. The GFP, in the form of a plasmid called pGLO, was added to a sample of E. coli and a control was created of E. coli without it. The E. coli was then placed…

The analysis of genetic markers for identification of biological evidential material in forensic science began with ABO blood group serology in the early part of the 20th century. The appreciation that individual genetic variation could be used to exclude suspected individuals from criminal activity propelled the analysis of other gene products including isozyme and polymorphic protein determination by slab gel electrophoresis. Forensic DNA analysis was introduced in the 1980s and became…

Introduction: In this lab experimentation, the group conducted Deoxyribonucleic acid isolation and restriction analysis on a plasmid from Escherichia coli cells. Plasmids are small circular DNA that are in the bacterium cells. Escherichia coli is a gram- negative bacterium that is known for variable reaction to antibiotics, and can be genetically manipulated. The gram- negative bacterium, Escherichia coli can be genetically manipulated by extracting a certain plasmid that allows it to resist…

Bacteria often contain plasmids, plasmids are circular, double stranded DNA that is not part of the nuclear DNA. Plasmids usually contain genes that code for several traits. Genetic transformation relies on inserting a gene that codes for a new trait in to the plasmid. For this lab, genetic transformation is the insertion of a new DNA in to E.coli cells, the pGLO plasmid was genetically engineered to carry the green fluorescent protein and a gene that codes for a protein that gives the bacteria…

Over the span of 35 years, our society has developed research towards Genetically Modified Organisms (GMOs).These organisms have provided many benefits to the society through nutritional value and over the years of being developed, there have been negative theories that have arisen in the eyes of skeptics. Although there are many legitimate concerns toward GMOs, labeling and regulating Genetically Modified Organisms can alert customers of the dangers and decrease negativity by informing them of…

Genetic engineering is an alluring and powerful branch of science and technology. The Merriam-Webster dictionary defines genetic engineering as, “the group of applied techniques of genetics and biotechnology used to cut up and join together genetic material...from one or more species of organism and to introduce the result into an organism in order to change one or more of its characteristics”. Genetic engineering has led to some of the most remarkable advancements in agriculture and medicine.…

Discussion When comparing the banding patterns of the crime scene to those of the suspects, the resulting gel indicates that Suspect 2 was at the scene of the crime. Although enzyme 1 produced identical DNA fragments across the gel, enzyme 2 did not. This is evident in lane D and possibly indicates that this enzyme was unable to bind to recognition sites similar to the crime scene DNA in well B. Thus, it produced a DNA fragment smaller in size that travelled further. Since the DNA evidence in…

Particles can move. One type of movement is diffusion. Diffusion is when particles move from an area of higher concentration to an area of lower concentration. The particles can move throughout a liquid, an area , or through a semipermeable membrane. Diffusion can be facilitated or non facilitated. When no other factors are involved in diffusion the particles are moving down their concentration gradient so that the amount of particles can be evenly distributed. if proteins or helpers are…

The result from the transformation process showed no colony growth on the agar plate, which means no plasmid DNA got inserted into the vector (Table 1). Thus, the transformation was considered unsuccessful. With that being said, the gene was not transferred among the bacterial cells. The reason could have been that the agar plate had a strong concentration of antimicrobial drug, so the bacteria may have been unable to grow under such condition. Another reason could be that the cell did not get…

d. This lab clearly demonstrated that cells maximize surface area to volume ratios. With a greater surface area, diffusion takes place over a larger surface and is much more effective. Cells rely on movement of particles through diffusion to carry out activities. If cells were too big, diffusion would take too long because although there is still a large surface in which diffusion can occur, the large volume catches up to the surface area and requires more diffusion. The .5x.5x.5 cm agar cube…