Molecular mass

Materials 1. Micropipette 2. Micropipette Tips 3. WARDS’S quick view DNA Stain 4. Prepared Agarose 0.8% 5. Tris-Borate-EDTA 5x Buffer 6. Electrophoresis Mold 7. Electrophoresis comb 8. Electrophoresis chamber 9. Electrophoresis power pack and cords 10. DNA from cow liver 11. DNA from chicken liver 12. 224 grams of fresh cow’s liver 13. 224 grams of fresh chicken liver 14. Meat tenderizer 15. Kitchen knife 16. Blender 17. 2.5 ml Salt 18. 31 ml dishwashing liquid (Dawn) 19. 354 ml warm water 20.…

DNA analysis by By Condy Kan Introduction Osamu Shimomura and his colleagues successfully discovered and isolated green fluorescent protein (GFP) from the jellyfish Aequorea victoria; GFP consists of a tripeptide, which is Ser-65, Tyr-66, and Gly-67, that forms fluorescence chromophore (1). Fluorescence chromophore, is accountable for its green fluorescence under UV light, helps to identify and determine the cells if the Enhanced Green Fluorescent Protein (EGFP) gene is expressed or not (2). In…

Professor, I found that in order to identify and distinguish any form of bacteria such as Serratia sp. and Enterobacter sp. a number of assessments should be conducted. Majority of bacteria’s has enzymes allowing it to break down nucleic acids and the bacteria and utilize the resulting nucleotides to produce their own nucleic acids. DNase is an enzyme that hydrolyzes DNA. The existence of DNase entails certain strains/species of bacteria and may be utilized for typing. A DNase assessment is…

When analyzing my DNA sequence I did not yield as much of a high quality sequences as others this may have been due to the fact of poor pipetting into the well or kit manufacturing issues. In order to find the changes that occurred in our DNA our reverse edited sequence was placed into James Licks mtHAP engine found at http://dna.jameslick.com/mthap/. Another search engine found on http://www.mitomap.org/foswiki/bin/view/MITOMASTER/WebHome was used to verify if the previous engine was correct,…

First lane is the protein ladder which is used as a reference and to estimate the size of different proteins. Lane 1 in the green box contains cow serum which shows high mobility since it dyes all the way to the bottom with different bands through the gel. Lane 2 contained one dark band at the bottom of the gel. Lane 3 shows o dark band with low mobility through the gel. Lane 4 shows one band stained with medium mobility through the gel. Sample proteins 1-4 were separated through gel…

Many proteins or nucleic acids have very similar charge to mass ratio andelectrophoresis of these macromolecules results in little or no separation of moleculesof different lengths, thus successful separation of proteins can be carried out invarious gels rather than in a liquid solution (Lodish et al., 2004). In…

Adult height is a polygenetic trait, which means that it is controlled by more than one gene. It is easily measured and highly heritable (h2 ~0.75- 0.9) (Kim, et al., 2010). Final adult height is the result of growth and development processes and has limited contribution from environmental factors (Weedon, et al., 2008). As a result, loci influencing height are compared and contrasted from different races. Genome-wide association (GWA) is a method used to quickly scan markers across genomes…

Introduction The group I would like to nominate for The ‘Carleton Prize for Biotechnology’ is comprised of three well-known scientists, James Dewey Watson, an American geneticist; Francis Crick, a British molecular biologist, and Maurice Wilkins, an English physicist and molecular biologist. The three worked together and achieved a significant impact on biotechnology in terms of the discovery of DNA structure (the double helix), and therefore they had been awarded Nobel Prize in 1962…

The Grim side of GMOs The ongoing debate around the world is, are genetically modified organisms, also known as GMOs, good for you and the environment? Research however shows GMO’s are bad for you, the environment and have had not enough testing. Imagine coming home, the whole day you've been waiting for that special treat in the fridge, nothing bad could happen from eating this treat on the outside, but inside your kidneys are slowly being destroyed and immune system weakened. These GMOs are…

DATABASES 1. Assembly Assembly database is needed to provide a systematic organization of genome. To accomplish that, the genome is fragmented into small pieces and is sequenced for many times to try to recreate the set of chromosome sequences. This database provides a unique tracker in form of accession so that users can identify changes to an assembly in precise locations whenever needed. There are two different types of assembly updates which are major release and minor release. 2.…