Molecular mass

The Sangers dideoxy sequencing method uses the dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. For this method to work effectively, it requires the following: a DNA primer, a single –stranded DNA template, radioactively or fluorescently labelled nucleotides, a DNA polymerase, and modified nucleotides that play a role in terminating the DNA strand elongation. The DNA sample is divided into four separate sequencing reactions, containing the DNA polymerase and all four standard…

III. Plans for Candidates Training and Research Career Development. a. Mentorship Plans to Enhance her Technical Repertoire. Dr. Rizvi will work in my laboratory, acquiring technical expertise in a wide variety of experimental approaches including use of genetically engineered mouse models of CCA, and patient derived xenografts of CCA (PDX). She will also become adept at cell biology approaches including confocal microscopy coupled with computer-based 3-D image reconstruction, expression and…

Bacterial Transformation with Escherichia coli Byanka Delgado BIO 181 Lab Thursday 1:30-4:15 Arizona State University Abstract The major objectives of the Bacterial Transformation Lab were to observe standard bacterial growth under various conditions including the transformation of bacteria; to understand how the process of transformation occurs, as well as the biological results and consequences that come of transformation. In order to correctly perform this lab, plasmids, agar plates,…

Protein X has a pI of 5.9, Protein Y has a pI of 7.6, and Protein Z has a pI of 8.5. For each of the methods below, describe how you would go about purfying Protein Y from Proteins X and Z (provide a pH that you would use and why.) Anion exchange chromatography: In anion exchange chromatography negatively charged ions will bind to the column. This will leave positively charged ions flushed through the column. By picking a pH of 6.5, protein X will flush through the column while protein Y and Z…

Abstract Plasmids are circular clusters of DNA that carry valuable genes that help a host survive. The process of transformation can occur and the genetic material of the cell can be altered with a plasmid. However, the cell must be competent so it has the ability to take in DNA from the environment in order for transformation to occur properly. The E.Coli was treated with CaCl2 in order for the cell to be competent. The presence or absence of ampicillin is used to test the E.Coli’s competence…

The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel. Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to the standard. Methodology: The experiment begins with the denaturing of the proteins through heating by test tubes as well as SDS buffer. This is accomplished…

The technique used in this experiment was serum protein electrophoresis to determine the levels of proteins in cow serum. The objective of this experiment is to perform a gel electrophoresis of cow serum by agarose gel electrophoresis and to interpret the banding patterns produced by the migration of the serum. Electrophoresis is the shift of ions in an electric field in which the protein moves in the gel based on size as well as in electric charge. The electrophoretic mobility of small…

The experiment aims to test the hypothesis that was raised in the last 60 years of the transformation principle by Avery, MacLeod and McCarty whom used the pneumococcal types. However, in this experiment the E.coli cells were used to determine the transformation principle. The strains were mixed properly with various enzymes under certain conditions of which cells can adapt, the participation of enzymes- Lysozymes, DNAse, protease and RNAse, and a buffer indicate the polymer in the cell that is…

The Great GMO Debate Introduction: Gmos have been introduced since the 1970s, and they have taken place of our main food supply. Although the creations of Gmos worry citizens about the unknown process of mutating our food supply these are just incorrect accusations while Gmos are beneficial for the stability of our society. The Debate on the Safety of Genetically Modified Organisms: In the last few decades Gmos have be introduced in order to fulfill our cultures supply needs for food.…

obtains forensic evidence. One of the main issues with the media influence today is how people assume that forensics in the real world is exactly like the shows that portray it. “If people’s reactions to crime and criminals are generally shaped by the mass media, then it seems reasonable to assume that public reactions to criminal cases are shaped by shows like CSI” (Tyler, 2006). These shows exhibit a perfect answer every time, with a nonexistent turnaround time on the evidence, and the best…