Molecular dynamics

The number of base pairs for the PGEX-KG (original) plasmid and PGEX-KG SAW1 (clone gene) is different. PGEX-KG- Saw1 has 794 bp more than original plasmid. The single digestion for both PGEX-KG and PGEX-KG-Saw1 will make a single cut in their respective restriction sites. The clone and original plasmids will become linearized and the PGEX-KG-Saw1 will be longer than PGEX-KG because of the insertion of Saw1. However, the insertion Saw1 can be isolated from the clone plasmid by doing double…

For the first week I performed some basic molecular biology techniques. I was putting GFP into plasmids and then translating them into E. Coli. This was preparation for the modular cloning experiment I performed in the second week. It was based on the golden gate model, allowing for a one tube digestion…

Change for a Longer Life Span Not a Better Span of Life. Everyone has different beliefs and thoughts about genetically modified organisms. Many believe that it is okay as long as the individuals ends up better than they were while others believe that an infant child should not be altered for any reason. What if a change in their genome can cause them to be immune to certain diseases, or even help cognitively with dyslexia? Would people still be opposed to something that can help an individual…

Pesticide resistant GMOs, and their effects on the wellbeing of the planet In an event as influential as the asteroid that ended the Cretaceous period, Homo-Sapiens began their work terraforming their world around them. They annihilated species while promoting other. They destroyed landscapes to fulfill their wants. They mixed and meddled into everything imaginable. Over the course of their actions they slowly tapped into the promoting force of life itself… Evolution. At first they bred animals…

The study of inheritance at the at one of the smallest levels is referred to as molecular genetics and is utilized for many purposes, which include genes, genotyping, sequencing, cloning, etc. (Kelly, 2016). This allows for the ability to conduct DNA analysis revealing varying compositions and properties. For the following experiment the genomic DNA from Drosophila melanogaster, was utilized to be cloned and sequenced for either actin or 18s rRNA. In order to clone and sequence the specific…

Literature published in the 1970’s document dioecious female Cannabis plants producing male flowers after treatment with colloidal silver (CS) during the flowering period (Sarath, 1979). While Van Der Straeten describes CS counteracting ethylene, a biosynthetic chemical expressed in female flowers, the gene expression changes from this treatment were not discussed (1991). What differential RNA expression occurs when transitioning female flowers to male flowers through CS treatment on Cannabis?…

Observation of mitosis in Onion root tips Answer the following question to its full explanation: 1. What is Cytokinesis? Compare and contrast cytokinesis between plant and animal cells. Cytokinesis is the last step in cell division, where the cytoplasm divide and the two daughter cells appear. In Animals, the cytokinesis processes occurs through cleavage. Firstly, in the old metaphase plate position, a cleavage furrow is formed. On each sides of the furrow, a contractile ring formed of actin…

Introduction: An electric toothbrush is a toothbrush that makes rapid automatic bristle motions, either back-and-forth oscillation or rotation-oscillation , to provide oral cleaning service, removal dental plaque from the surface of teeth. Electric toothbrush generally powered by rechargeable battery or disposable battery. The functional unit for the electric toothbrush is 3 years per use where 2 times per day. The functional unit for the replaceable brush head is 3 months per use. We will…

Dmitri Iosifovich Ivanovski (1864-1920) was a famous botanist, plant physiologist, and microbiologist (Lechevalier, 1972) who lived in Russia. He is considered one of the founders of virology. Ivanovski also did work with tobacco plants, mosaic disease, soil microbiology, and fermentation. Ivanovski’s life was very interesting. His early years were spent in a small village in Russia. In his later life he went to different universities and participated in many scientific experiments, lectures,…

Kulwant originally wanted to be a plant breeder and had an interest in plant genetics. He loved plants and wanted to do research in developing new plant varieties. Therefore, he got a Masters of Science in Plant Breeding. After University, he worked as a Research Assistant in Pulse Breeding. Then he had to leave his job after only working 9 months to immigrate to Canada. Unfortunately, when he got here he couldn’t find any jobs related to plant breeding, so he was forced to do odd jobs to make a…