Molecular dynamics

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    Name: Maryam Sadiqa Group Members: Oralia Reyes Anny Then Anthony Mayers Date of lab performed: 06/4/16 Date of submission: 06/23/16 Lab Name: Genes in Human Population lab Page number: 57-66 Purpose of the lab: The purpose of this lab was to work with a partner and identify your partners and as well as your own phenotype and genotype. We did that by deeply observing our bodies and then determining whether we have the following traits. PTC, Darwin’s ear point, ear lobe attachment,…

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    Retinitis Pigmentosa is an inherited retinal disease with progressive degeneration of the photoreceptors, leading sometimes to blindness, it is well characterized by its heterogeneous genetics. The first signs can be observed in adolescence or early adulthood including night blindness and progressive loss of peripheral vision in daylight, eventually concluding in severe visual impairment, may be inherited as X-linked, autosomal dominant and autosomal recessive. Many genes had been involved with…

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    Human FOXP2 Gene Analysis

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    gene evolved in such a manner that it is the genetic basis of the evolutionary development of language neural circuitry. As discussed earlier, downstream targets of the transcription factor were identified, but there were only suggestions for the molecular pathways that were up and down regulated. Further analysis using comparison of the expression in FOXP2 to FOXP2chimp found specific accelerated highly conserved non-coding sequences in the human version and used whole-genome micro arrays to…

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    Polymerase Chain Reaction

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    In week one Polymerase chain reaction (PCR) was used to amplify RTKs that were derived from thoroughly smashing five fruit flies and was combined with 1 ml Buffer A (630 ul ddH2O, 100ul 1M Tris/Hcl pH7.6, 200 µl 0.5M EDTA, 20 µl 5M NaCl, and 50 µl 10% SDS), then incubated for 15 minutes at 65˚C so that as much DNA could be secluded as possible. 200 ul of KAc/LiCl working solution, 1 part 5M KAc (potassium acetate) and 2.5 parts 6M LiCL (lithium chloride), mixed and chilled for a full 10 minutes.…

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    Dna Mapping Lab Report

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    Edgar Huichapa DNA Mapping Lab 4-29-15 Analysis of Plasmid DNA by DNA Mapping Introduction Restriction mapping is the process of obtaining structural information on a piece of DNA by the use of restriction enzymes (Restriction). Restriction enzymes, also known as restriction endonucleases, are naturally occurring enzymes used in labs today to cut DNA into smaller, more manageable fragments (Bougher). Restriction enzymes are used to cut plasmids which are small DNA molecules that can replicate…

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    difficult to concentrate due to broadening of the bands of the eluting molecules through the column. While both techniques of chromatography achieve in separating the components within a mixture, the size exclusion chromatography helps determine the molecular mass of the separated compounds and the affinity chromatography is helpful in yielding the highest concentration of protein purification due to its…

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    Pig Micropigs

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    An article in the Smithsonian describes how scientists in a Chinese genomics institute used a common gene editing enzyme called TALENs to create micropigs, which weigh about 33 pounds at maturity. These micropigs were first created to study the human stomach bacteria and new medications, which can help answer questions about the human body itself. By using an enzyme to stop the pigs from growing, the researchers altered their DNA, which would then be passed down to their offspring. This is…

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    Potato Diffusion Lab

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    IV. Discussion The Concentration Gradients is known as known as diffusion. They are spontaneous process because there is no energy input at the beginning (Reece et al., 2011). Each substance could diffuse to its own concentration gradient without affected by the concentration of other substances (Scott 2015).In the first experiment of the Concentration Gradients. The color of the clear water was expected to change into the color of the potassium permanganate dye, which was purple. Observation…

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    Rhiju Das came to Butler on March 21st to give a lecture on how a videogame has helped code for RNA. Diseases can be traced back to RNA molecule folds. RNA vaccines have a design problem because it is hard to tell what sequence is correct. NUPACK 08 was the most sophisticated computer system and it got the sequence wrong. However, Taipan was designed by a videogamer and it had the correct sequence. Therefore, Das designed a game called Foldit in which players solved puzzles of computers. A…

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    incubated in caffeine, the DNA strands need to be split. We will use a lysin to deconstruct the DNA and then place it on a gradient. After this we will place the gradient in a motor and rotate the DNA. This way the DNA will be fractionated and then the molecular weight can be calculated. Another possible experiment could be to take L cells and soak…

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