Molecular diffusion

To start this experiment, two microcentrifuge tubes were exposed to different ingredients. One was labeled “+DNA,” the other with “-DNA.” They were both filled with different bacteria. More specifically, the “+DNA” tube had pGFP in it and included a plasmid. Included on that plasmid were two genes: gfp (gene for green fluorescent protein) and an ampicillin resistant gene. The gfp gene makes cells glow and the resistant gene fights against ampicillin, which kills cells. This plasmid DNA was…

What is cloning? Coning is the process of making a replica of something using its DNA. How does it affect agriculture? One way cloning affects agriculture is animals. The perfect animal can be made through cloning. Take a farm animal for example. You take the DNA from one farm animal, and clone it. A cow for example, it would never be sick, if it was a milking cow, it would produce some of the best milk. If it was a meat cow, you would get the best beef. If a female, it would get pregnant with…

Paul Berg’s method for inserting foreign genetic information into DNA of SV40 was truly a paradigm shift. No longer was the study of molecular biology purely observational, Dr. Berg’s discovery gave scientist the traction to synthetically modify and transduce foreign DNA into a desired host. Because of this, not much was known about this type of genomic modification. Except for the knowledge…

A DNA ladder is a solution of DNA molecules of different but known length. In Well 1, the DNA ladder was applied to the gel to set a standard for the rest of the samples. In LoggerPro, after setting an origin, the second step was to set a standard ladder. We standardized the ladder by going from 4 to 5 cm and determining that was 10 milliliters. The next step was to scale the ladder by selecting the 3 brightest points and labeling it 3000,1000,500 in descending order. The estimated size of my…

the components in the products, documenting the processes and results as the research was actively conducted. I am currently interested in developing my knowledge and research abilities in the fields of Virology, Gene Therapy, Biochemistry, Molecular and Cellular Biology, Microbiology, and Immunology. My primary area of interest lies within the study of microorganisms and their ability to cause devastating illness in Eukaryotic organisms. In my academic career, I have always been…

Throughout the previous couple of decades, and as far back as numerous grown-ups can recall, the perspective of gene inheritance has been composed in the four letter dialect of DNA. The American Association for the Advancement of Science said that the hereditary changes and re-combinations have driven most portrayals of how phenotypic qualities are passed on starting with one era then onto the next. Analysts and doctors endeavored to unravel and find the pieces of information that recommended…

Materials 1. Micropipette 2. Micropipette Tips 3. WARDS’S quick view DNA Stain 4. Prepared Agarose 0.8% 5. Tris-Borate-EDTA 5x Buffer 6. Electrophoresis Mold 7. Electrophoresis comb 8. Electrophoresis chamber 9. Electrophoresis power pack and cords 10. DNA from cow liver 11. DNA from chicken liver 12. 224 grams of fresh cow’s liver 13. 224 grams of fresh chicken liver 14. Meat tenderizer 15. Kitchen knife 16. Blender 17. 2.5 ml Salt 18. 31 ml dishwashing liquid (Dawn) 19. 354 ml warm water 20.…

DNA analysis by By Condy Kan Introduction Osamu Shimomura and his colleagues successfully discovered and isolated green fluorescent protein (GFP) from the jellyfish Aequorea victoria; GFP consists of a tripeptide, which is Ser-65, Tyr-66, and Gly-67, that forms fluorescence chromophore (1). Fluorescence chromophore, is accountable for its green fluorescence under UV light, helps to identify and determine the cells if the Enhanced Green Fluorescent Protein (EGFP) gene is expressed or not (2). In…

Professor, I found that in order to identify and distinguish any form of bacteria such as Serratia sp. and Enterobacter sp. a number of assessments should be conducted. Majority of bacteria’s has enzymes allowing it to break down nucleic acids and the bacteria and utilize the resulting nucleotides to produce their own nucleic acids. DNase is an enzyme that hydrolyzes DNA. The existence of DNase entails certain strains/species of bacteria and may be utilized for typing. A DNase assessment is…

When analyzing my DNA sequence I did not yield as much of a high quality sequences as others this may have been due to the fact of poor pipetting into the well or kit manufacturing issues. In order to find the changes that occurred in our DNA our reverse edited sequence was placed into James Licks mtHAP engine found at http://dna.jameslick.com/mthap/. Another search engine found on http://www.mitomap.org/foswiki/bin/view/MITOMASTER/WebHome was used to verify if the previous engine was correct,…