Molecular diffusion

In addition, a cell’s life depends on cell transport. Osmosis is the diffusion of water, which controls the movement of water in and out of cells. Osmoregulation regulates the water concentration in cells to maintain homeostasis, which is very important for our bodies and living organisms. When cells go through osmosis, their…

Figure 2. The proteomic organization of three subfamilies of Smads (Co-Smad, R-Smad and I-Smad) and organization of a Smad MH1 domain with DNA. (A) All Smads information taken from PDB entry Smad>UniProt Gene list of Smads. The conserved N-terminal MH1 domain is in red, linker region in dark blue and the C-terminal MH2 domain in deep yellow. In the linker region the red PXS/TP (or S/TP) indicates the potential phosphorylation site for MAPKs ERK1/2, and the square indicates the PY…

C5-curcuminoid-4-aminoquinoline based molecular hybrids: Design, synthesis and mechanistic investigation of anticancer activity Shamseer Kulangara Kandi,a Sunny Manohar,a Christian E. Vélez Gerena,bBeatriz Zayas,b Sanjay V.Malhotrac* and Diwan S. Rawata* Received (in XXX, XXX) XthXXXXXXXXX 20XX, Accepted Xth XXXXXXXXX 20XX DOI: 10.1039/b000000x Abstract: Theprivileged scaffolds of curcumin and 4-aminoquinolines are extensively used in the design and synthesis of biodynamic agents having…

Imagine the potential of copying the DNA of a creature exactly, to create a replica of that creature. Opening a door to a multitude of scientific research. Now imagine the repercussions that could come from tampering with the natural cycle of life. Both seem intimidating, intriguing, yet convoluted. Since the first successful cloning experiment in 1996, a sheep named Dolly, there was is question that cloning is a force to be reckoned with. The experiment posed new opportunities and also…

B D E C O S Y S T E M 2 0 1 5 Petersohn, D. ; Spencer, M. ; Fratila, A. ; Chi-RenShyu Present a novel method by using an in-memory computing environment database for all mammals in Ensembl release 80 for identifying, extracting, and indexing palindromes in a searchable. [27] Rathore, M.Mazhar ; Ahmad, Awais ; Paul, Anand ; Daniel, Alfred Proposes a set of requirements for obtaining pervasive, integrated information system of EOS and associated services like real-time and offline data…

Protein Purification Practical Report Student Name : Noel Murphy Date : 28 February 2015 UF/DF Practical Question Report Ultrafiltration (UF) and Diafiltration (DF) steps are carried out prior to every chromatography step and post the final polishing chromatography step. These UF and DF steps are vitally important, the UF step concentrates the protein product into a smaller volume of buffer, while the DF step alters the conditions the protein product is sitting in i.e. Buffer exchange.…

Q1. Explain segmental trans-splicing (STS) and mention a long gene 8 kb or more, this strategy could be used for. A1. Segmental trans-splicing concept is similar to that of trans-splicing, where exons of two different gene fragments are joined together. However, in STS it can be used to target gene sequences that are too large for a viral vector genetic capacity; for example the von Willbrand factor gene (8.6 kb) or the muscular dystrophy gene (11.0 kb). In STS a 5’exon of the gene is delivered…

A. Polymerase chain reaction (PCR) Principle It includes the primer mediated enzymatic amplification of DNA. It uses the ability of DNA polymerase to manufacture new strand of DNA complementary to the offered template strand. DNA polymerase can add a nucleotide only onto a preexisting 3′-OH group to add the first nucleotide that is way Primer is required. Then DNA polymerase elongates its three ends by adding more nucleotides to generate an extended region of double-stranded DNA.…

Recombination and mutation are the motivation of evolution. Recombination is critical for repairing DNA lesions and for chromosomal pairing, and exchange during meiosis (Krejci et al. 2012). Recombination does not occur uniformly on the chromosomes of eukaryotes. Meiotic recombination in well studied yeast revealed that non-uniformity of recombination was observed when the frame of reference is an entire chromosome, multigene region and a pair of genes or a small region upstream of a gene…

Transformation is the process by which competent bacteria take up genetic material (in the form of naked DNA) from the environment. In this lab, the process of transformation was conducted by using a plasmid containing various genes called pGLO along with the bacterium Escherichia coli. In order for transformation to occur in Escherichia coli, repeated washes in the presence of CaCl₂ followed by heat shock treatment (42°C for 50 seconds) occurred to induce the competence of the bacterium.…