Molecular diffusion

A Variable Number Tandem Repeat (VNTR) is a molecular motif that plays a considerable role in molecular genetics and specifically forensic analysis [1]. The goal of this experiment is to observe the tandem repeats of the VNTR (D1S80) locus through molecular lab techniques. Cheek cells were isolated from an individual and amplified via a polymerase chain reaction (PCR). Then, the amplified sample was separated through gel electrophoresis. This project entails the interpretation of fluorescing…

The orthologs of candidate genes in QTL regions and their annotation in Arabidopsis were obtained from the result of BLASTn based on gene sequence. Some genes related to seed weight from papers and some searched from Arabidopsis database (https://www.arabidopsis.org/index.jsp) using key words “seed weight”, “seed size”, “cell number” and “endosperm growth”. Homologues were clustered and analyzed using the Database for Annotation Visualization and Integrated Discovery (DAVID) tool, an online…

Background info: DNA is in everyone’s body. So how would you tell the different DNA of each person from each other? Sure DNA is unique, but how can you measure it and look at it? If you were able to see the DNA cut up, could you tell from the pieces and sizes , which DNA belongs to which person? You can, using gel electrophoresis. Gel electrophoresis separates DNA fragments in accordance to their size. In the place of DNA, you could use food coloring to see if it works. With gel electrophoresis,…

In 2005, new sequence technique, which known as next-generation or second generation sequencing, was emerged after three decades of Sanger sequencing dominance. The new technique, 454 pyrosequencing, has completely new mechanism compared with the previous one, where this technique depended on series of enzymatic steps that including four enzymes polymerase, ATP sulfurylase, luciferase and apyrase under pyrosequencing mechanism. The name 454 pyrosequencing derived from pyrophosphate that realised…

Hannah Noetzel Blk. 3 10/25/17 Bacterial Transformation Utilizing E. Coli Background Through this lab there were many terms used that may confuse you so before I begin explaining the lab, here’s some background information on the subject of biology. A plasmid is bacteria naturally containing one or more tiny circular pieces of DNA. The two genes being used in this experiment are Ampicillin and fluorescent genes. When reading about the lab, the most common thing used in…

Gel electrophoresis is used to separate DNA, RNA, and protein molecules by using an electric field. Protein gel electrophoresis is similar to agarose gel electrophoresis, but runs protein bands instead. The technique of protein gel electrophoresis uses cross-linked polymers of acrylamide (which is a neurotoxin and requires careful handling) or polyacrylamide to separate proteins and small nucleic acids. This technique takes longer than the agarose gels used to separate larger nucleic acids. Also…

Plasmids are extra chromosomal DNA within some bacteria that exist as circular structures. Although they are not essential for survival of the bacterium, they often do confer many environment advantages such as antibiotic resistance. One bacteria of interest is E. coli, a gram-negative organism that is part of normal human gut flora. Some strains of E. coli are pathologic to humans, however some strains are beneficial. For example, one strain produces vitamin K, an essential nutrient. In this…

metastatic potential and an even worse prognosis than pancreatic adenocarcinoma[1, 2]. A major hindrance towards development of therapies against pancreatic ASC is that the molecular mechanisms underlying its pathogenesis remain poorly characterized. In this proposal I will take advantage of the expertise on RNA biochemistry and molecular biology that I have acquired during my doctoral training and combine it with the in vivo approaches developed by the Ventura lab to investigate the potential…

Literature Review: Green Fluorescent Protein and Histidine Tagged Proteins Histidine and green fluorescent protein (GFP) tagging are important in molecular biology because they allow for purification, tracking, and quantification of target proteins (Ferrari et al., 2004; Cho et al., 2011; Deponte, 2012). Histidine tagging provides a method for isolating and increasing the amount of target protein recovered from a biological organism or mixed sample of proteins (Masek et al., 2011; Singh and…

RESULTS Transformation: the transformation of the ade2 gene to the kanamycin resistant gene (ade2::kan2R) cause the cell to become red and grow on mediums containing G418 or kanamycin resistant mediums was observed to have occurred. PCR and Gel Electrophoresis: Figure 1 is the product of gel electrophoresis containing the wild type and transformed PCR products, either being or not being cut by HindIII. From the left (reader’s left) of the gel to the right the lanes are; 1.Ladder, 2. transformed…