Molecular biology

Restriction Mapping of Plasmid DNA Preparing 5 Eppendorf Tubes All tubes contain 1 μL of the plasmid DNA, and 2 μL of 10X buffer #2. Tube 1 is the control so there is no enzyme added only 17 μL DI water. For tube 2 the DNA is mixed with 0.5 μL of BamHI and 16.5 μL of DI water, to see total number of base pairs from the linear DNA. In tube 3 DNA is digested with 2 restriction enzymes, which can give the relative distance between the two enzymes. To be more specific, 0.5 μL of Bam HI and EcoRI…

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One thousand- It seems to be a fairly large number, but in some cases, especially mine, it can be extremely small. The average person has from one hundred and fifty thousand to four hundred thousand blood platelets, yet there are times where I found myself with as low as one thousand of them. It was in times like those were I looked up to courageous people like Jackie Robinson, who faced difficulties head on with no fear. I quietly sat in the airport boarding gate with my eyes screwed shut,…

Quavian Belton Biochemistry I Dr.Nicholas Panasik October 6, 2015 PCR Optimization Abstract A Polymerase Chain Reaction (PCR) is a reaction that is set to catalyze the amplification of a copy of a piece of DNA. The aim of this lab was to optimize a PCR reaction by finding out the optimum concentration of MgCl2 needed. Six reactions were set up four with varying concentration MgCl2 and two primer control groups. The volumes and concentrations of each component of the PCR…

1. Where do restriction enzymes come from and what is their original purpose? Restriction enzymes come from bacteria. Bacteria used restriction enzymes to cut up DNA into fragments to fight off viruses. 2. a. Why do you get different fragment sizes if you cut the DNA from two different species with the same restriction enzyme? Each species has a different arrangement of bases, and the restriction enzyme only cuts after a specific order of bases. Due to that being the case, the specific…

A plasmid is circular, relatively small in size, double stranded as a DNA molecule, and is physically distinct from the chromosomal DNA of a cell. Innately found in prokaryotic bacterial cells, plasmids can sometimes be found in eukaryotes as well 1. Plasmids are self-replicating; therefore, when a plasmid is formed, infinite copies of the plasmid can be created if the plasmid is grown in bacteria. 2 Because of the size of plasmids, which can range anywhere from one thousand base pairs up to…

I was keenly aware of the importance of Genetics in health care early in my time studying Medicine and later have gained profound knowledge of it as well as Cell Biology and Molecular Biology through in-depth Biomedical studies. However, it is during my current study on the genetic disorder Neurofibromatosis type 1 (NF1) when my passion for the Laboratory Genetics and Genomics was lighted. My passion for the Laboratory Genetics and Genomics was lighted while I am conducting current studies on…

Earth. The intricate relationship between molecular, anatomical and physiological characteristics of it astounds me, yet what interests me most is not how powerful the human body is as a whole, but how ultimately our physiology can fail us. Studying biology at an undergraduate level will allow me to grasp a deeper understanding as to why this happens, as well as develop my existing knowledge of the mechanisms involved in keeping humans alive. A-level biology has provided me with a broad…

Forensic Functioning In America the word forensics proposes crime in a way that excites stories on serial killers, murder, crime scene investigation, and heavy DNA analysis. In reality, this only breaks the ice on what forensic science fully consists of. Forensic science is initially any science used for the purpose of law. The evidence tested is used in the court of law, criminal investigation, and trial. A forensic scientist does perform tests on blood and bodily fluid as well as…

When you walk into a laboratory of biology, you will see people recording, discussing, and testing. Biology is a science that based on thinking and testing of a theories. Scientists of biology will join together to gather and information in the laboratory, forming a discourse community. A discourse community is a group of people who have common interest and have methods for communicating ways to achieve those goals. According to author Gary D. Schmidt and William J. Vande Kopple in “Discourse…