Molecular biology

Electrophoresis; Separation of Proteins Purpose: To create an electrophoresis gel in order to further separate Cytochrome C, Myoglobin, Albumin and Hemoglobin(proteins) based off of their net charges. Materials: • Rabbit hemoglobin • Serum albumin(cow) • Myoglobin • Cytochrome C • Microtubes • Tape • Micropipets • Agarose gel • Buffer • Gel chamber • Test tubes • Test tube holders • Gloves • Macropipets Properties of Proteins: Protein Color Isoelectric Point Net Change at pH 8.6 Cytochrome C…

In 1969, Thomas Brock and Hudson Freeze of Indiana University discovered a new bacteria species in the Yellow Stone Park, that scientist thought it could not survive above 55 degrees Celsius. Thermus aquaticus is a typical gram-negative bacterium, meaning that its cell walls contain much less peptidoglycan and unlike gram-positive bacteria, gram-negative bacteria contain lipoproteins. T. aquaticus appears as either a rod or short filaments. When exposed to sunlight, it can exhibit a yellow, pink…

It has been proven that yeast can grow on both YPD and SD plates. In this lab we cross several yeast strains through the process called replica plating. For this identification test only one dominant allele is required. One copy of each gene is essential to produce enough of each enzyme needed in order to make a histidine. Through the study of Saccharomyces cerevisiae we prove that by transferring yeast cells onto YPD and SD plates over a period of 24-72 hours in a thirty degrees Celsius…

Illustration of sexduction in E. coli using streptomycin resistant F-lac- recipients and F′lac+ donors Abstract The process in which DNA from a donor cell is transferred through physical contact into a recipient cell is known as conjugation. In the experiment, sexduction, a cross between F′ and F- plasmids, was demonstrated by mixing the donor and recipient, diluting them and using selective plating methods. The plasmids from the donor, recipient and transconjugant were isolated and analyzed…

The Cremation Process, Explained Cremation refers to the combustion, oxidation and vaporization of a corpse to basic chemical compounds, including gases, mineral fragments and ashes. Following cremation, the family of the decedent receives a container or urn that holds their loved one’s ashes. Many times, for financial reasons, an individual will forego a funeral with a burial, opting for cremation: The National Funeral Directors Association (NFDA) states that since 2005, cremation rates have…

Cloning is a cell, cell product, or organism that is genetically identical to the unit or individual it is derived from. During the process of cloning, a piece of DNA is cut from its source with a restriction enzyme. Then, the DNA is put into a vector ( a DNA molecule used to transport genetic material into another cell). The vector is introduced to a host cell by the process called transformation, then the host cell copies the vector DNA with the original DNA; creating copies of the inserted…

Imagine having glowing parts at night in your body. Having the ability to glow in the dark would be fun. There are many things in the world that glow in the dark. For example, glow sticks, lightning, fireworks, mushrooms, bacteria, algae and many more. The Aequorea Victoria Jellyfish is a jelly that glows in the dark. I am more than interested to learn how luminescence works and its processes. The Aequorea Victoria Jellyfish is a jellyfish found in the Puget Sound, Washington State. The…

between identified species. Electrophoresis Electrophoresis of DNA run in 2% agarose gel showed successful extraction of DNA in samples 1-8, 12 and 14-16. Samples were of good quality with high molecular weights (Figure 2). Samples 11 and 13 in Row 1 of gel 2 were not easily visible and thus the molecular weight was difficult to…

Highlands Ranch, CO, June 22, 2012 Dr. Chad Prusmack, through his nutraceutical company PruTectRx, has been authorized by T.A. Sciences to prescribe the telomerase activator, TA-65. Popularized by Suzanne Somers in her book, Bombshell: Explosive Medical Secrets that Will Redefine Aging, TA-65 has been shown to lengthen telomeres in DNA strands, which can help slow aging, hinder the development of cancer and decrease genomic instability. TA-65 is only available through authorized,…

1.1 Caenorhabditis elegans (C. elegans) Caenorhabditis elegans is a free-living non-parasitic, non-infectious, non-pathogenic, transparent Nematode (roundworm). It has a full grown length of about 1mm, a life span of around 3 weeks and is found in temperate soil environments where it survives by feeding on microbes such as bacteria. [1] Consisting of about 1000 somatic cells as an adult, C. elegans is amenable to genetic crosses and produces a large number of progeny, at times even…