Molecular biology

Certain groups of people work very well at high altitudes. People who live at higher altitudes are expected to have a higher hemoglobin count since hemoglobin helps deliver oxygen to tissues. The amount of oxygen in the atmosphere decreases as the altitude increases. The increase in hemoglobin helps blood cells carry more oxygen to nourish the body. This is true with the majority of people who live in these regions such as in the Andes, Rockies, and the Alps. There is a group of people from…

DNA (deoxyribonucleic acid) is the genetic material that carries the genetic instructions being used in growth, development and reproduction of all living things. DNA has regions called genetic polymorphisms that are used to determine paternity/maternity, kinship, determination of human remains, and inherited diseases. DNA is an example of what forensic use for evidence in a crime. Using regions in the DNA you can determine a “fingerprint” for an individual that can help in a crime, with this…

Previously we were taught that our DNA is what make us unique. But what if I told you that our DNA didn’t do all the work? There is a protein in our bodies that makes our DNA coding complete and it’s called an epigenome. There are two proteins that make up one protein of a epigenome and they are histones. They are wrapped around the DNA and the Methyl group that are attached to it. Of course we are taught that our DNA makes up who we are as far as characteristics; However, epigenetics explain…

Thus, the identification of LITAF localization pattern in Dictyostelium throughout its development will offer valuable insight into the protein’s function. Furthermore, I intend to also develop a LITAF gene knockout in Dictyostelium using various molecular techniques such as RNA interference, PCR amplification, cloning, transformation and Southern blotting. A gene knockout study will further elucidate LITAF function by allowing the identification of abnormalities during growth and development…

Fingerprint evidence is highly accurate in the identification of suspects, because the likelihood of two different people possessing the same set of fingerprints is estimated to be one in 64 billion (Hall, 2015). Therefore, state and federal courts universally accept fingerprint evidence during a criminal trial due to the validated science behind the analysis of fingerprint characteristics (Hall, 2015). Next, as with everything else in law enforcement, computers have advanced fingerprint…

The Sangers dideoxy sequencing method uses the dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. For this method to work effectively, it requires the following: a DNA primer, a single –stranded DNA template, radioactively or fluorescently labelled nucleotides, a DNA polymerase, and modified nucleotides that play a role in terminating the DNA strand elongation. The DNA sample is divided into four separate sequencing reactions, containing the DNA polymerase and all four standard…

Assignment #2: The Replication of DNA in Escherichia Coli Due Date: Feb 8, 2016 at 9:00 AM CST 1a. A procedure in which a small amount of DNA material is immersed in concentrated solution, cesium chloride (CsCl), and soon after placed in centrifuge until the point where equilibrium is achieved is called density-gradient centrifugation. Contrasting techniques involving diffusion and sedimentation form a persisting CsCl concentration gradient. Furthermore, the concentration gradient and pressure…

Rizvi will work in my laboratory, acquiring technical expertise in a wide variety of experimental approaches including use of genetically engineered mouse models of CCA, and patient derived xenografts of CCA (PDX). She will also become adept at cell biology approaches including confocal microscopy coupled with computer-based 3-D image reconstruction, expression and use of fluorescence-based bioprobes, gene silencing and editing technology, immunofluorescence and immunohistochemical approaches,…

Bacterial Transformation with Escherichia coli Byanka Delgado BIO 181 Lab Thursday 1:30-4:15 Arizona State University Abstract The major objectives of the Bacterial Transformation Lab were to observe standard bacterial growth under various conditions including the transformation of bacteria; to understand how the process of transformation occurs, as well as the biological results and consequences that come of transformation. In order to correctly perform this lab, plasmids, agar plates,…

Protein X has a pI of 5.9, Protein Y has a pI of 7.6, and Protein Z has a pI of 8.5. For each of the methods below, describe how you would go about purfying Protein Y from Proteins X and Z (provide a pH that you would use and why.) Anion exchange chromatography: In anion exchange chromatography negatively charged ions will bind to the column. This will leave positively charged ions flushed through the column. By picking a pH of 6.5, protein X will flush through the column while protein Y and Z…