Molecular beam epitaxy

The purpose of this experiment is to examine the extraction of the DNA material that was extracted from the Chromosomal DNA from E coli in laboratory 9. DNase is used to help extract the Sugar phosphate backbone of the DNA. DNase should only cleave DNA not RNA but DNase can classify contaminated RNA from chromosomal DNA. Methods A. preparation :E. coli was used to extract the DNA as instructed in laboratory 9 to use in the experiment on analyzing in this experiment. There was 500 μL of the…

Certain groups of people work very well at high altitudes. People who live at higher altitudes are expected to have a higher hemoglobin count since hemoglobin helps deliver oxygen to tissues. The amount of oxygen in the atmosphere decreases as the altitude increases. The increase in hemoglobin helps blood cells carry more oxygen to nourish the body. This is true with the majority of people who live in these regions such as in the Andes, Rockies, and the Alps. There is a group of people from…

DNA (deoxyribonucleic acid) is the genetic material that carries the genetic instructions being used in growth, development and reproduction of all living things. DNA has regions called genetic polymorphisms that are used to determine paternity/maternity, kinship, determination of human remains, and inherited diseases. DNA is an example of what forensic use for evidence in a crime. Using regions in the DNA you can determine a “fingerprint” for an individual that can help in a crime, with this…

Forensic Functioning In America the word forensics proposes crime in a way that excites stories on serial killers, murder, crime scene investigation, and heavy DNA analysis. In reality, this only breaks the ice on what forensic science fully consists of. Forensic science is initially any science used for the purpose of law. The evidence tested is used in the court of law, criminal investigation, and trial. A forensic scientist does perform tests on blood and bodily fluid as well as…

due to the repulsive forces between the negatively charged phosphate groups the nitrogenous bases would be located on the inside and the phosphate substituents on the outside (Crick). This monumental discovery fueled the creation of new forms of molecular biology specifically aimed at knowing the action of a gene. James Watson was born in Chicago in 1928; where he also received both his B.S. and Ph.D. in Zoology from the University of Chicago. In 1951,Watson’s first postdoctoral year, he…

Previously we were taught that our DNA is what make us unique. But what if I told you that our DNA didn’t do all the work? There is a protein in our bodies that makes our DNA coding complete and it’s called an epigenome. There are two proteins that make up one protein of a epigenome and they are histones. They are wrapped around the DNA and the Methyl group that are attached to it. Of course we are taught that our DNA makes up who we are as far as characteristics; However, epigenetics explain…

Thus, the identification of LITAF localization pattern in Dictyostelium throughout its development will offer valuable insight into the protein’s function. Furthermore, I intend to also develop a LITAF gene knockout in Dictyostelium using various molecular techniques such as RNA interference, PCR amplification, cloning, transformation and Southern blotting. A gene knockout study will further elucidate LITAF function by allowing the identification of abnormalities during growth and development…

Fingerprint evidence is highly accurate in the identification of suspects, because the likelihood of two different people possessing the same set of fingerprints is estimated to be one in 64 billion (Hall, 2015). Therefore, state and federal courts universally accept fingerprint evidence during a criminal trial due to the validated science behind the analysis of fingerprint characteristics (Hall, 2015). Next, as with everything else in law enforcement, computers have advanced fingerprint…

The Sangers dideoxy sequencing method uses the dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. For this method to work effectively, it requires the following: a DNA primer, a single –stranded DNA template, radioactively or fluorescently labelled nucleotides, a DNA polymerase, and modified nucleotides that play a role in terminating the DNA strand elongation. The DNA sample is divided into four separate sequencing reactions, containing the DNA polymerase and all four standard…

Assignment #2: The Replication of DNA in Escherichia Coli Due Date: Feb 8, 2016 at 9:00 AM CST 1a. A procedure in which a small amount of DNA material is immersed in concentrated solution, cesium chloride (CsCl), and soon after placed in centrifuge until the point where equilibrium is achieved is called density-gradient centrifugation. Contrasting techniques involving diffusion and sedimentation form a persisting CsCl concentration gradient. Furthermore, the concentration gradient and pressure…