Molecular beam epitaxy

The inhibitor DDX3 used in our laboratory was Human shDDX3 of lentivirus, and the control group was shLuc of lentivirus, which was transfected into the cells by infection. First, BHK-21 cell was cultured to 6 cm dish and cultured to a cell density of about 12%, then remove the medium and rinse twice with 1 ml of PBS. After removing the PBS, then add lentivirus (shDDX3 and shLuc) (MOI = 50) and 3ml 2% RPMI (containing 1/1000 polybrene), then culture for 24 hours at 37 ℃ with 5% carbon dioxide…

Abstract This experiment was a study of the different properties of a lipid bilayer. We used fluorescent microscopy to study the lateral diffusion of lipids and binding properties biotin and anti-biotin. The effect cholesterol has on the fluidity of the membrane was tested using Fluorescent Recovery After Photobleaching (FRAP). We used a Nikon Eclipse 50i upright fluorescent microscope with a Digital Sight DM-2MBW CCD camera to take images of the bilayer after photobleaching. Using these images…

The four forces of evolution are mutation, gene flow, genetic drift, and natural selection. Mutation is when a change happens during replication of DNA. It is when the copy of the original DNA is not replicated completely correctly. There are multiple types of mutation including substitution, deletion, duplication, insertion, nonsense mutations, missense mutation, frameshift, and repeat expansion. Another force of evolution, gene flow, also called migration is exactly that.…

DNA sequencing (Sanger to NGS) Introduction: The DNA sequencing methods have quickly evaluated for the forty last years and their finding was joined by breakthroughs in the field of Molecular biology (1). The first efficient method to sequencing DNA was found by F.Sanger in 1977 (2). But the huge desire to sequencing genomes required faster methods and many improvements were made. Fluorescence, capillary electrophoresis and microarrays led to a new way for sequencing genes : the Next Generation…

Ammonium sulfate precipitation, ion exchange chromatography, and SDS-Page were the three different techniques used in this experiment. The standard molecular weight of rubisco is 66 kDa and 14 kDa. rubisco was expected in P2 with nothing added. Also in P2 medium salt the evident of rubisco. T The standard coefficient of rubisco is between 14.1 ug/mL and 18.2 ug/mL (Hudson, 1994). The average of the two…

Eukaryotes have much larger genomes than prokaryotes, and therefore, must condense their DNA into chromatin. Chromatin is composed of histone proteins that help to condense and organize the DNA forming chromosomes. The basic unit of this chromatin is a nucleosome, which contains about 150 base pairs of DNA that are wrapped 1.7 times around the core histone proteins. However, this tightly wrapped chromatin becomes a problem when regulatory proteins need access to specific sections of the DNA.…

BIOL 1010 ASSIGNMENT 1 OCT 6 BY JORDAN KAPITANY ST 100883963 Among the many scientific achievements of the twentieth century in the field of bio-technology scientists Paul Berg, Herbert W Boyer, Stanley N Cohen and team for their research that lead the party to discover a technique of taking genes from one organism and inserting them into another organism, also more formally known as Deoxyribonucleic Acid or DNA recombinant technology. In 1971 Berg and team successfully isolated DNA of…

Lynchel Brumaire DNA, the genetic code of all organisms, can help in the analysis of the organisms evolutionary history. All organisms, alive and extinct, descended from one creature. As time went on mutations accumulated in the DNA sequence of the progeny. By sequencing the genetic code of an organism in relation to another, their evolutionary history can be placed onto a phylogenetic tree. In this experiment, DNA barcoding was used to to identify a species and place it onto a phylogenetic tree…

A single Lactaid pill was grinded with a mortar and pestle. 120 mg of the ground up Lactaid pill was dissolved in 200 mL of 0.08 M phosphate buffer (7.7 pH) and the resulting solution was centrifuged to remove any capsule remains. Enzyme and Substrate Solution Preparation A blank tube was prepared by adding 5 mL 0.08 M phosphate buffer (7.7 pH) and 0.5 mL of 2.5 nM ONPG to a cuvette to zero the spectrophotometer with the spectrophotometer set to 420 nm. The substrate solutions were prepared…

C.R.I.S.P.R.-Cas9 is a technology that is used to target specific stretches of genetic code and edit DNA at precise locations.It also finds breaks in the DNA and either puts it back together with a tiny change, or puts it back together with a piece of donor DNA. It allows scientists to modify genes in living cells and organisms, and also may cure mutations in the future. I think that this technology is and isn’t a good thing to spread around the world. There is more pros to C.R.I.S.P.R.-Cas9…