Molecular beam epitaxy

Objective: In-silico assessment analysis of Protein database entries of the Pf. lactate dehydrogenase (PfLDH) enzyme, considered as an important target for designing of anti-malarial drugs. Methods: PDB structures of the enzyme Pf. lactate dehydrogenase were downloaded from Protein Data Bank. These were subjected to ANOLEA energy assessment analysis and Swiss model was used to analyse Ramachandran plot. Results: The energy assessment analysis and analysis of Ramachandran plot were carried out…

Preparation of coating solutions Chitosan solution was prepared with 2 % (w/v) chitosan in 1% (v/v) acetic acid glacial. The solution was placed on a hot plate/magnetic stirrer for 6 h. The resultant chitosan-based coating solution was filtrated, through a Whatman filter paper No: 2, glycerol was added at 0.75 % (w/v), and stirred for more than 15 minutes. Two of the best concentrations, 60 and 90 mg mL−1 were prepared from freeze-dried IgY for S. putrefaciens and P. fluorescens respectively.…

Bovine serum albumin (BSA) is usually used for generation of nanoparticles (NPs) in drug delivery systems. This paper presents a study on the simple coacervation method for fabrication of BSA nanoparticles.An artificial neural network (ANN) model was developed to predict the diameter of nanoparticles based on experimental data. Effect of pH, temperature, initial BSA and cross linker concentration, molar ratio of BSA to ethanol, agitation rate and rate of ethanol addition were investigated on the…

genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genetic engineering has applications in medicine, research, industry and agriculture and can be used on a wide…

Evaluation When performing the experiment, the pill bugs were generally unresponsive, and many stayed in the same place throughout the whole experiment. Outside of this, no major problems occurred while performing the experiment. If the experiment were to be performed again, more of the IV (baking soda, rubbing alcohol, etc.) would be added to produce a more pronounced effect on the pill bugs, as most pillbugs did not move at all. The results are mostly trustworthy and accurate, as the data is…

Venous blood samples (~5 ml) were collected from normal healthy subjects (n-10) and patients presenting with NSTEMI (n=50) on admission. Based on the duration between the onset of chest pain symptoms and hospital admission, samples were divided into three groups such as hospital admission within 1 hour ( ≤1 hr), or after 1 hour but within 4 hours ( ≥1 hr -≤4 hrs) and after 4 hours but within 8 hours (≥4 hrs- ≤8 hrs) after the onset of chest pain. Half of the blood sample was allowed to stand…

A. F. Hess (New York) and A. Windaus (Gottingen, Germany) tested 30 different steroids with more than 1 double bond from various plant sources for antirachitic activity. Upon irradiation they found that ergosterol , a fungal steroid from ergot which when irradiated , was highly active in curing rats suffering from rickets14. In consultation with Rosenheim and Webster they also determined the ergosterol was the provitamin D which was convertible to vitamin D by UV irradiation15. In 1931 Reerink…

Determining the Amount of Protein in Each Homogenate: Hepa-1 cells were infected with many EYFP expression plasmids that had be previously homogenized and denatured in Laemmli buffer. The protein samples were diluted to a concentration of 1μg/μl. The different tubes are labeled as follows: GOL for golgi, TUB for tubulin, ACT for actin, MIT for mitochondria, and HEP for non-transfected Hepa-1 cells. SDS-PAGE: A gel was obtained, then while wearing gloves the package was cut open and gel was…

it is impossible to tell what her paternal haplogroup is without knowing what her father’s paternal haplogroups are, while men have a Y chromosome which makes it possible for their paternal haplogroup to be found. Everyone has Mitochondrial DNA so both men and women can find their maternal haplogroup, however, you will need to conduct a test to find out. To conduct a test, you may need a subscription to Ancestry.com, or pay for 23andMe. When you receive your DNA kit, you will have to provide a…

New technological advances occur every day. These advances have begun to spill into most everything in modern society, such as agriculture, consumer health, the environment, food production, medicine, and much more. One of these advances is genetic engineering and the organisms that scientists genetically engineer called genetically modified organisms, or GMOs. Today, there are many controversies over GMOs and whether or not they are good for society due to the process and possible repercussions…