Molecular beam epitaxy

In week one Polymerase chain reaction (PCR) was used to amplify RTKs that were derived from thoroughly smashing five fruit flies and was combined with 1 ml Buffer A (630 ul ddH2O, 100ul 1M Tris/Hcl pH7.6, 200 µl 0.5M EDTA, 20 µl 5M NaCl, and 50 µl 10% SDS), then incubated for 15 minutes at 65˚C so that as much DNA could be secluded as possible. 200 ul of KAc/LiCl working solution, 1 part 5M KAc (potassium acetate) and 2.5 parts 6M LiCL (lithium chloride), mixed and chilled for a full 10 minutes.…

Edgar Huichapa DNA Mapping Lab 4-29-15 Analysis of Plasmid DNA by DNA Mapping Introduction Restriction mapping is the process of obtaining structural information on a piece of DNA by the use of restriction enzymes (Restriction). Restriction enzymes, also known as restriction endonucleases, are naturally occurring enzymes used in labs today to cut DNA into smaller, more manageable fragments (Bougher). Restriction enzymes are used to cut plasmids which are small DNA molecules that can replicate…

difficult to concentrate due to broadening of the bands of the eluting molecules through the column. While both techniques of chromatography achieve in separating the components within a mixture, the size exclusion chromatography helps determine the molecular mass of the separated compounds and the affinity chromatography is helpful in yielding the highest concentration of protein purification due to its…

An article in the Smithsonian describes how scientists in a Chinese genomics institute used a common gene editing enzyme called TALENs to create micropigs, which weigh about 33 pounds at maturity. These micropigs were first created to study the human stomach bacteria and new medications, which can help answer questions about the human body itself. By using an enzyme to stop the pigs from growing, the researchers altered their DNA, which would then be passed down to their offspring. This is…

IV. Discussion The Concentration Gradients is known as known as diffusion. They are spontaneous process because there is no energy input at the beginning (Reece et al., 2011). Each substance could diffuse to its own concentration gradient without affected by the concentration of other substances (Scott 2015).In the first experiment of the Concentration Gradients. The color of the clear water was expected to change into the color of the potassium permanganate dye, which was purple. Observation…

Rhiju Das came to Butler on March 21st to give a lecture on how a videogame has helped code for RNA. Diseases can be traced back to RNA molecule folds. RNA vaccines have a design problem because it is hard to tell what sequence is correct. NUPACK 08 was the most sophisticated computer system and it got the sequence wrong. However, Taipan was designed by a videogamer and it had the correct sequence. Therefore, Das designed a game called Foldit in which players solved puzzles of computers. A…

incubated in caffeine, the DNA strands need to be split. We will use a lysin to deconstruct the DNA and then place it on a gradient. After this we will place the gradient in a motor and rotate the DNA. This way the DNA will be fractionated and then the molecular weight can be calculated. Another possible experiment could be to take L cells and soak…

chromatography column and according to the results from Part III that appears to be the case. Glucose oxidase is made up of two subunits each having a molecular weight of about 80 kilo-Daltons. The third fraction, which contained the last elution material, had a band on the SDS-PAGE gel at around 72 kilo-Daltons. Although alkaline phosphatase has a similar molecular weight it also consists of two subunits making each subunit only 40 kilo-Daltons. Unfortunately the gel bands for wells 8 and 9…

PCR History The invaluable molecular technique known as PCR, or the polymerase chain reaction, was discovered by a biochemist named Kary Mullis. Mullis was doing research in the early 1980s at a biotechnology company in California when he initially thought of the idea regarding PCR. PCR was developed by a combination of several techniques already in existence; the synthesis of oligonucleotides and their utilization to synthesize new copies of DNA that were specific using DNA polymerases. Mullis…

E. coli Genomic DNA Isolation and Quantitation Introduction In this experiment, the genomic DNA of E. coli cells were isolated, purified, and quantified. To achieve this, E. coli cells were lysed, purified from impurities and Methods E. coli cells were first centrifuged in order to separate the cell pellet from the supernatant. Cell pellets were resuspended in saline-EDTA, which weakens bacterial membranes and chelates metal ions. Since E. coli has three layers (outer membrane,…