Microtiter plate

The anti-benzoylecgonine (cocaine) monoclonal antibody [IP3G2] (mouse IgG1, catalog no. GTX44358, lot 821400072, 3.75 mg/ml) was purchased from Gene Tex (Irvine, CA, USA). The second anti-benzoylecgonine monoclonal antibody [IP3G2] (mouse IgG1,catalog no. MAB4029, lot 8H23323, 3.75 mg/ml) was purchased from Acris Antibodies (Herford, Germany). The secondary antibody, a polyclonal anti-mouse IgG from sheep (catalog no. R1256P, lot 21481, 2.2 mg/ml), was obtained from Acris Antibodies (Herford,…

Plant Material Collection Fully grown whole plants of Tribulus terrestris Linn. were collected from different localities of Chennai, Tamilnadu. The fruits were separated out from the plants and washed in running tap water to remove adhering dirt and soil particles and dried first under shade in room temperature and secondly in a hot-air oven for complete drying at 50-60˚C. The dried fruits were made into coarse powder by grinding and stored in labelled bottles for further study and…

Inflammation is a defense mechanism where the body’s white blood cells fight off bacteria, viruses, and damaged cells(Feeling the Heat- the Link between Inflammation and Cancer, 2013) . When pathogenic cells enter the body, the white blood cells surround that invasion site immediately(Feeling the Heat- the Link between Inflammation and Cancer, 2013) . The white blood cells then release a specific toxic chemical that denatures the pathogen enough to kill it(Feeling the Heat- the Link between…

describes: antibody was coated on the plate, incubated, washed and blocked with BSA. Again plate was washed, sample and IL-6 was added, incubated, washed and detection antibody was added, incubated and washed. Avidin- HRP was added, incubated and washed. SuperAqua Blue solution was added and absorbance was measured. Use of substances:In ELISA, bovine serum albumin (BSA) was used as a blocking agent to avoid non-specific binding of antigens and antibodies to the microtiter wells. Washing buffer…

INTRODUCTION: Over prescription and extensive use of antibiotics has caused antibiotic resistance at an shocking rate (Mezger et al.2014). The main aims of Antibiotic sensitivity testing is detecting possible drug resistance in common pathogens by in-vitro testing of bacterial cultures with antibiotics to determine susceptibility of bacteria to antibiotic therapy. The tests are frequently used when the etiologic agents are members of species capable of resistance to commonly prescribed drugs…

13. What is the process resulting in antigen and antibody moving toward each other? One dimensional diffusion Enzyme-Linked Immunosorbent Assay 14. In the “sandwich” analogy of the direct ELISA, what is the “bread”? Antibodies What is the filling? Antigens 15. Describe some advantages of using the ELISA technique to process a large number of samples. The advantage of Sandwich ELISA is that the sample does not have to be purified before analysis,…

Introduction: Antibiotic sensitivity testing is used in clinical laboratories to predict the effectiveness or failure of antibiotics in vivo by testing in vitro. The need for antibiotic sensitivity testing is urgent due to the surge of antimicrobial resistant pathogens in recent years. Antimicrobial resistance hinders the prevention and treatment of the infections caused by parasites, bacteria, fungi and viruses. Antimicrobial resistance is the resistance of a pathogen to an antimicrobial that…

Capture anti-CRP Ab can be polyclonal from goat, sheep, rabbit, etc., and commercial sandwich ELISA kits comprise of capture anti-CRP Ab-bound microtiter plate (MTP) or Ab-bound 96-well plate strips for coating the MTP, apart from CRP standards, biotinylated anti-CRP detection Ab, and horseradish peroxidase (HRP) conjugated to streptavidin (SA-HRP). The commercial TMB (3,3 ',5,5 '-tetramethylbenzidine) substrate kit…