Microtiter plate

We first evaluated whether Honokiol treatment was toxic to the APP-CHO cell line. Cells were treated with various concentrations of Honokiol (0.5 μM, 1 μM, 2 μM, 5 μM, 10 μM and 20 μM) for 24 h and cell survival was determined by Prestoblue® assay (Fig.1A). As shown in Fig.1A, when exposed to Honokiol concentrations of 10 μM or lower, the viability of APP-CHO cells was the same as untreated control cells. However, a significant decrease in cell viability was observed with 20 μM Honokiol…

The End Zone: Measuring Antimicrobial Effectiveness with Zones of Inhibition Introduction: A Kirby-Bauer test is a microbiological method used as a form of testing bacterial resistance, it’s also known as a Zone of Inhibition test (1). The way in which the Zone of Inhibition was determined in this experiment was by measuring the diameter of the resisted zone in 3 areas and taking an average. As numerical data was required for this it means that in this experiment the Zone of Inhibition Test was…

Change is contradictory. Change can be as predictable as the tides rising and falling against the beach. It can also be as unpredictable as an earthquake that splits the ground and swallow homes whole. Many may try to resist the turn of the tide or the rumble of the earth, but there exists no way to avoid the inevitable. In a South Africa divided by apartheid, the old minded tribe struggles to strike a delicate balance with the new white rule with neither side willing to give into the other’s…

Mount St. Helens is an 8,363-ft. tall volcanic landmark of the state of Washington. It was a popular tourist attraction and recreation area for most of the 20th century. It was also The U.S.’s 8th tallest mountain, towering at 9,977 ft. It is now the 14th tallest at 8,363 ft. tall, due to a major eruption in May of 1980. On May 18, 1980, the beautiful, peaceful well-known mountain Mount St. Helens became the most dangerous mountain in U.S. history in a matter of minutes. There are records of…

from the famous board game Monopoly. However, I only took the concept and instead of playing for money the object of the game was to test your knowledge about tectonic plate boundaries. When I tried the game out in class I was not sure which approach I would take as far as should the game be weather related due to tectonic plates, or different catastrophes…

1.1 Juan de Fuca Ridge - case study In Salmi et al. (2014) they deployed thermal blankets in a 450-700m area of a segment on the Juan de Fuca Ridge to examine the effects of hydrothermal circulation on very young oceanic 2 crust. The Juan de Fuca Ridge is a tectonic spreading center located off the coast of Washing- ton in the United States. The ridge is considered an intermediate spreading ridge as it spreads 5.6-5.7 cm/yr.
Several methods exist to measure the heat flow, some measure the…

Introduction I will be discussing the similarities and differences between uniformitarianism and Catastrophism. The debate on these being going on for years. Why these two theories are very different from each other. Catastrophic believe that there was a great worldwide flood. Uniformitarians believe that the world started from the Big Bang Theory. How old is the Earth thousands of years old or billions of years old. Both theories have their own compelling arguments on how…

Lahar is an Indonesian word with its English alternative or equivalent being Lava. That said, it suffices to say Lahar is a volcanic mudflow. That being the case, for a flow to occur, there has to be a slope or a driving force, otherwise it will be stagnant. Because a slope is involved you would expect lahars to be ubiquitous in mountainous terrain. The best case scenario to depict how a Lahar looks like is to picture a mixture of concrete and water moving down a slope at a great speed…

I believe that there are no plate tectonics on Venus. I believe this because of the reasearch that i did on this topic. Venus shows no evidence of plate tectonics on it's planet. " There are no long, linear volcano chains or any clear subduction zones. The transform plate boundary, causes the plates to slide past one another, which may cause earthquakes or fault lines, which is exactly what is happening. The plates are sliding past one another and earthquakes ar occuring which may lead one to…

Method: Purpose: To investigate how Porcellio Scaber reacts in terms of orthokinesis to different humidities in their habitat. Equipment-  75 slaters which range from 5-10mm in length (kept in a plastic container with holes in the lid, with dirt and bark so that they are kept in a natural environment as possible so can react naturally during experiment.)  5x petri dish set ups labelled from 50-90% (One petri dish on the bottom will contain glycerol and have a mesh/gauze hot glued over top…