Microscopy

MITOCHONDRIAL SRUCTURE Mitochondria can be seen in the light microscope, but their detailed internal structure is only revealed by electron microscopy. As ubiquitous, semi-autonomous cellular organelles, mitochondria are separated from the cytoplasm by the outer and inner mitochondrial membrane.The outer membrane is porous and freely traversed by ions and small, uncharged molecules through pore-forming membrane proteins (porins), such as the voltage-dependent anion channel VDAC [19]. Any larger…

Hypothesis 1: pMN progenitor fate is biased by the position of the cell within the pMN domain. Experimental Approach: Transgenic olig2:EGFP;actb2:mCherry donor embryos cells were transplanted into stage-matched wild-type hosts. Confocal time-lapse microscopy was used to image individual EGFP+ mCherry+ pMN cells continuously until 42 hpf, assess the behaviors of individual progenitors within the pMN domain. Division of the cells was…

coatings treated with SPS at 800 jC and up to 6 min. Furthermore, changes in microstructure was made and found that the SPS-induced WC reconstruction can apparently be achieved within the coating surface with limited thickness. Transmission electron microscopy (TEM) results also showed the evidence of supplementary reaction between Co and WC/W2C to form Co3W3C during the SPS processing. Microhardness values obtained on the surface of SPS-treated coating showed 40% enhancement over as-sprayed…

Recently there has been a rising interest in studying social microbial behavior, which can provide fundamental information about the development of severe infections in host organisms by pathogenic bacteria. These microorganisms demonstrate highly social behaviors such as the ability to communicate by producing, releasing, detecting and responding to small signaling molecules. This process, named quorum sensing (QS), allows a bacterial population to regulate its population’s behavior in response…

Table 1 Composition of Lansoprazole microparticles Formulation code Amount of drug(mg) Amount of polymer chitosan (mg) Drug polymer Ratio Tween 80 (%w/v) F1 100 100 1:1 1.25 F2 100 200 1:2 1.25 F3 100 300 1:3 1.25 F4 100 400 1:4 1.25 F5 100 500 1:5 1.25 F6 100 600 1:6 1.25 Characterization of Lansoprazole microparticle The microparticle will be characterized for following parameters: Percentage yield: After complete drying, the drug loaded microparticle was collected and weighed accurately…

diffusion distance, and proportion of wrapping were manually measured for vessel pairs that were matched in both the light micrographs and micro-CT images in a blinded manner. Artery-vein pairs were identified, matched in both micro-CT and light microscopy images, and then labelled. Measurements were first made for the artery-vein pairs in the light micrographs. Then, measurements were made for the artery-vein pairs in the micro-CT images. A total of 25 vessel pairs were identified across two…

overnight, embedded in paraffin, sectioned at 5 μm, mounted on clean glass slides, and stained with hematoxylin and eosin (H&E) for histological evaluation. Each slide was coded and analyzed in a blinded fashion by two investigators using light microscopy. Each sample was assigned a histological score based on cumulative scores assigned to colon characteristics including degree of epithelial damage, loss of crypt architecture, inflammatory cell infiltration, and edema. For each category, a score…

Title: Exploring the Factors Affecting Post-Wound Hair Regeneration in Mice. Hypothesis: We hypothesize that in mice large wound size increases the number of hair follicle because large wound will activate the Wnt pathway that is necessary for hair follicle regeneration in wounds. Independent variables being tested: The wound size of the mice. Dependent variables being tested: The number of hair follicle. Experimental Design: This experiment will be designed to determine if large wound size,…

Introduction Quantum dots (Q-Dots) is very small semiconductor particles that means it can be either conductors or resist electricity depending on the temperature and purity of the semiconductor. Q-dots range in size from 2-10 nm, so because of their small size, quantum mechanics governs the physics of the particles. The synthesis Q-Dots were first studied in 1982 by Efros and Ekimov, and then many developments have reported the synthesis of semiconductor nanoparticles.1 Q-Dots have unique…

P. aeruginosa is a gram-negative non-sporulating bacillus that belongs to the phylum of Proteobacteria. It can adapt and thrive in different niches, ranging from water/soil to plant/animal tissues. Even though it is commonly found in the environment, it frequently causes infections in plants and animals (1). Luke (1862) was the first to report P. aeruginosa in infections as he observed rod-shaped particles in blue-green pus of some infections. This coloration is a hallmark of P.…