Microscope slide

stain procedure was the first test performed by isolating a colony from the pure culture streak plate. The gram stain was followed orderly right from the referenced laboratory manual. The test resulted to be Gram- Negative bacteria from the compound microscope which were pink and rod shaped that can be seen with the 100x objective lens with the oil immersion. After approval from the professor, the first part of the unknown was to isolate all the gram positive bacteria since the unknown…

The Uinta Mountains are known for their great appearance of life and its tranquility. This can be seen just a couple of feet away from the well known Mirror Lake Highway. While I am here, I witness multiple coyotes, beaver dams, deer, cows, and much more. The geographical features are outstanding as well. Magnificent, clear lakes stood in sequence one after the other in the mountains. Any traces of wind shatter the lakes of their once untouched state. Also, the Provo River slices its way through…

Cannulae implantation: For stereotaxic implantation of Cannulae, mice were anesthetized with 1.0-1.5% isoflurane and secured in a Kopf stereotaxic apparatus. Bilateral stainless steel guide Cannulae (26G, Plastics One) were targeted for placement 1.0 mm above the center of the Anterior Cingulate Cortex (ACC) (AP: +1.5 mm relative to bregma; ML: ±0.4 mm; DV: −0.5 mm from the surface of the skull) or 1.0 mm above the center of the prelimbic subdivision (PL) (AP: +1.7 mm relative to bregma; ML:…

Materials: Test tube, Pasteur pipette, centrifuge, microscope, glass slide, normal saline, Anti-Human Globulin (AHG), Coomb’s Control Cells (CCC) and Anti-D. Methods: 1. 5 % cell suspension of the patient’s red cell in saline is prepared. 2. 2 drops of Anti-D is added to a test tube marked as ‘Test’ 3. Two drop…

Trichomoniasis is a sexually transmitted disease that affects the reproductive system. It is a very common yet curable STD among young sexually active men and women. Although women are more generally affected by this disease, men are able to become infected and pass it on as well. This particular type of STD is caused by a small single-celled parasitic organism called Trichomonas vaginalis. The parasite is oval in shape with four hair like strands protruding out of it which helps to propel the…

Impact of penicillin, novobiocin, and kanamycin on Staphylococcus aureus bacterial growth Introduction: In today’s world, bacteria are becoming more abundant and strong in terms of their resistance to antibiotics. As a result of increasing prescription potency, clinicians are supposed to be conscious of those patients who are more inclined to infection by prescribing the appropriate antibiotics on the basis of their sensitivities along with their cultures (Fleming et al., 2006). A recent case…

Isolated Colonies and Soil Samples A. Purification of Serratia marcescens and Staphylococcus epidermis The objective was to grow isolated colonies on a nutrient agar medium (NA). Many microorganisms found in soil are able to grow on this medium. Since there are many organisms that are found in the soil, only a few of this organisms are adapted to live in this specific medium obtained. The NA plate is the first way to isolate microorganisms because the organisms that will be left on the plate…

that helps your physician find out if a fungus is causing your rash. The physician takes a sample of your skin by lightly scraping the infected area. The skin sample is placed on a slide with a potassium hydroxide solution and is gently heated. If the fungus cells are present then the doctor can see them using a microscope. A Wood’s light exam is used to see if the rash is caused by a fungus. The physician shines a special light on the skin where you have the rash. Only certain types of fungi…

their ability to bind the safranin. Gram-positive microorganism, however, stains blue due to their ability to retain the primary stain, crystal violet. I determined my unknown sample to be a gram-negative microorganism. After viewing the slide under the microscope, I concluded that my unknown gram-negative colony has small microbes that were rod shaped. The following week, we conducted three tests. Initially, we inoculated an enterotube to determine the identity of our unknown…

CHAPTER 4: IDEA GENERATION & EVALUATION In order to measure pitch accurately we came up with 5 ideas. In the end we analyzed all those ideas in terms of following criteria’s 1. Ease of use 2. cost 3. Accuracy 4. Ease of fabrication. 4.1 Vernier Calipers method The Digital Caliper (sometimes incorrectly called the Digital Vernier Caliper) is a precision instrument that can be used to measure internal and external distances extremely accurately. The example shown below is a digital caliper as…