Microscope slide

chamber was then prepared to be put under the microscope, by filling it with ice and a small volume of water. This was done to ensure the temperature would remain around 15 degrees Celcius, which could keep Daphnia magna efficiently cool. Furthermore, the different concentrations were prepared in separately labeled eppendorf tubes, by adding distilled water to the 50 mM of caffeine to dilute them to the appropriate concentrations chosen. The slides were then put on the cooling chamber, after…

The purpose of the research was to potentially find a new bacteria that is capable of producing an antibiotic against one or more of the six ESKAPE pathogens (a group of pathogens that are resistant to most if not all current antibiotics). All research protocol was based on the Yale Small World Initiative and the third edition of Microbiology Laboratory Theory & Application. One bacteria from a sample of soil collected (colony #18) was tested for its ability to produce antibiotics against two of…

All of the isolates shown in Table 1 were resistance to S. aureus and were considered antibiotic producers. However, only isolate 12 and 16 showed resistant to the unknown Gram-negative organism. All of the antibiotic producers were then tested against Enterococcus faecalis, Pseudomonas putida, Acinetobacter baylyi, Escherichia coli, and Enterobacter aerogenes by the streak-plate method, which can be seen in Table 2. As demonstrated in Table 2, none of the antibiotic producers were found to be…

Title: To investigate the reproductive structures of the plants and to understand its importance in the survival of plant species. Introduction: Angiosperms are the dominant form of plant life in most terrestrial ecosystems, comprising about 90 percent of all plant species. Most crop and ornamental plants are angiosperms. Their success results, in part, from two innovative structures: the flower and the fruit. Flowers are derived evolutionarily from modified leaves. The main parts of a flower…

Mix together the unknown stock culture into the hydrogen peroxide drop. Observe the absence or presence of bubbling. KOH (Potassium Hydroxide) Test: Place a drop of 3% KOH on a slide. Use a toothpick and transfer a good amount of the bacteria to the droplet of KOH. Stir it together. Raise and lower the toothpick off the slide to observe the results. Obligately aerobic organisms will grow on top but not in the stab. Motility Agar: Obtain a motility agar tube. Using an inoculation pick stab 2/3 of…

1) What is a PCV? What does PCV stand for? What is another name for PCV? Describe the procedure for obtaining a PCV. o PCV stands for packed cell volume. o Another name for a PCV is hematocrit. o A PCV is the measurement of the ratio of packed red cells in a known volume of whole blood sample. o The procedure is to take blood from your patient and put it into a purple top tube, which has the anticoagulant EDTA in it. • Next you need to fill 2 micro hematocrit tubes with the blood about ¾ a…

Abstract The purposes of this experiment are to become familiar with the anatomy and behavior of Planaria and make a design to test which aspect of diffusion gradient model which will cause development. The body parts of planaria was observed under a microscope. The flatworms were cut into 3 parts equally based on the gradient model . After a week, the new body parts were observed to have regenerated into new species. The hypothesis was rejected because the results did not match the hypothesis…

Title: Ecological Interactions of Living Organisms in Beaver Creek Statement of the Problem: How do the organisms in Beaver Creek interact with the biotic and abiotic portions of the ecosystem? The sample was taken from Beaver Creek (a tributary of Tookany Creek) in Glenside, Montgomery County, PA. The pH of the creek was 5.5, and the stream velocity was detectable (greater or equal to walking speed). In the area of collection, the stream width was one meter and the depth was 14 cm. There…

instance, heating the slide too long could cause it to break. If the inoculation loop is too hot before handling the specimen it can distort the cell shape (Harley, 2014). Timing of the chemicals is also important to Gram staining. These chemicals are crystal violet, gram iodine, decolorizing, and safranin. However, if the chemicals are not followed correctly it can cause a negative reading and will show a distortion in the cell shape (Harley, 2014). Over staining of the slide can cause a…

24-48 hours. A 6.5% Salt Broth Test was conducted by obtaining a tube of 6.5% salt broth, inoculating the broth with the unknown, and incubating it at 37C. For a catalase test, a small amount of unknown was transferred onto a slide. A drop of H2O2 was then added to the slide, and observations were recorded. A Mannitol Fermentation test was then conducted by obtaining a Mannitol Salt Agar (MSA) plate. The unknown was streaked onto the plate and incubated inversely at 37C. Next, a Coagulase test…