Microscope slide

Introduction Plant reproduction is a fascinating part of biology. Plants, specifically angiosperms, have unique ways of reproducing. Angiosperms use pollen vectors such as insects, mammals, birds, wind, and even water to deliver their pollen grains to stigmas. The plant of study for this experiment was Mimulus guttatus, also known as the monkeyflower. This plant produces yellow flowers that are bilaterally symmetrical and have five petals. Interestingly, their petals are fused together and…

is because of pathogenic strains that can be potentially harmful to the public and ourselves. Typically, microorganisms are identified through various streak plate methods and under a microscope. The streak plate method helps isolate wanted pure colonies of bacteria to observe their characteristics under the microscope. But how does one tell the difference between different strains of bacteria of the same genus and species? One method that is used in the realm of foodborne pathogens is through…

The slides were blocked using 5% Bovine serum albumin in Tris buffered saline-Tween 20 (TBST) for 1 h at room temperature to avoid non-specific binding. Then, the specific primary antibody for PCNA (IHC grade) and GST-pi (1:800 dilutions) were added to the sections and incubated at 4◦C, overnight. After washing, the slides were incubated with secondary antibody IgG-HRP (1:1000 dilutions) in TBS and 0.05% Tween 20…

Ionic and Covalent bonds are the two different types of bonds that hold atoms together, each having their own characteristics. The distance and charge determines the type of bond the molecules have. These bonds occur because atoms strive to obtain the ideal valence of 8. Some atoms need to gain or lose electrons in order to achieve the ideal valence. In a covalent bond, the electrons are being pulled by two atoms that are both nonmetals. In other words, the two atoms are sharing electrons, but…

test were conducted, first a gram stain test was performed in order to determine the unknown’s gram reaction and its cell morphology. After the gram stain procedure the unknown bacteria appeared purple and roughly spherical, when seen through the microscope. Thus, this concluded that the unknown bacterium was gram positive and its cell morphology was cocci. After this, a series of test were conducted in order to identify the unknown. The…

Yogurt The General Method (Second Class Time) 1. We spread 3 kinds of bacteria on the slide using a toothpick and a drop of water. 2. For each specie we used different toothpicks and put three water drops. 3. By using an alcohol flame lamp we fixed the bacteria to the slide. 4. Added a drop of the fuschin to each of the three species and warmed the slide over the alcohol flame. 5. We did destain (D) the slide by using a few drops of acid-alcohol. 6. Used the gentle steam of water to rinse…

stick. Sample is removed and is now grinded down to 30 m to become translucent and therefore become visible under microscope. 5.2.2 Preparation of a dyed sample Sample is cleaned and cut into a square of size 2X2 cm. Dye is prepared using 95 mg epoxy Resin, 0.3 mg blue Dye, 12.5 ml thickener, 12.5 ml thinner Epoxy and hardener mixture is prepared (used to glue samples to the glass slides). Mixture - 4 mg epoxy and 1 mg hardener. Sample is placed in a container and is placed in vacuum to remove…

CONTENTS PAGE To be completed at the end. BACKGROUND Endoparisites live inside its host mainly inhabiting the stomach as it is the perfect condition. The most common and dangerous endoparasite to sheep in Australia is the barbers pole worm. These specific worms thrive in warm moist, conditions and especially after rainfall. These blood sucking worms can grow up to 20- 30mm and female worms are capable of laying up to 10 000 eggs a day. The barber’s pole worm has three main life…

Unknown Bacteria 228 Adisney Pino Professor Dowding Miami Dade College Section T/R 7:05PM Introduction Enterobacter aerogenes is rod-shaped bacteria that belongs in the family of Enterobacteriaceae. This rod-shaped bacterium is gram negative which makes it a facultative anaerobe which grew perfectly in 37-degree temperature. This species was motile and therefore it can synthesize an enzyme known as ornithine decarboxylase. This bacterium is prevalent in the intestines of animals…

purple and the Gram-negative bacteria should be the pink color of the counterstain. The staining procedure is visualized in Figure 1.1 below. Figure 1.1 Part 1 involved the preparation of a slide containing the above bacterial species, E. coli, B. subtilis, S. epidermidis, and M. catarrhalis. A backup slide was prepared in case a proper stain was not achieved, but was not used. Part 2 involved the preparation of a Gram-positive species, a Gram-negative species, and Unknown #2, which was…