Introduction Life could not exist without microorganisms: plants depend on microbes to obtain soil and cows need microorganisms to digest cellulose. Microbes degrade waste and they live in our bodies to keep us healthy. Microorganisms are also used to make vaccines and antibiotics. However, some bacteria are known for causing diseases such as the common cold, AIDS, Herpes, and Chicken Pox. Knowing the identity of microbes is essential for knowing the causative agent of a disease in order to…
used a light microscope with an HDMI port attached to a computer. We photographed both controlled and affected samples three times each. The slide images were saved onto the laptop and sent to the group member’s emails. To examine the results from the DRP1 gene inhibition, the cells had to be injected with mito-GFP. Mito-GFP is a green fluoresces gene from jellyfish. The GFP insertion allowed us to examine the mitochondria in the cells with a fluorescence microscope. This microscope takes three…
Centro College Abstract This experiment studies the effect of caffeine and alcohol on heart rate. This lab experiment detailed how water flea, (Daphnia magna) a transparent aquatic organism whose heart can be easily identified using a compound light microscope, was used to study the effects of caffeine and alcohol on heart rates. According to the research alcohol was observed to have caused a decrease in the heart rate of Daphnia magna while caffeine caused an increase in the heart rate.…
Desulfovibrio is gram negative bacteria. There are media that will be useful in isolating this organism. The best option for a gram negative bacteria would be using a selective media specifically MacConkey agar or EMB agar. These agars are used to isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a…
Materials: Microscope, glass slides, coverslips, lens paper, Tetrahymena culture, 20 µL micropipette, Detain©, timer, calculator, Neff (growth medium), distilled water, 50 mM salt water, 500 mM salt water Procedure: Follow the given procedure. Variables: The independent variable…
Then Christmas came, and my parents got me my first science kit. Oh, I was so happy I couldn’t control it. The kit had a microscope, a real scalpel, prepped slides and slides that I can make. I played for hours with it, I even made my own blood slide with the help of my dad of course. So it was safe to say that this sparked my passion for science. All during high school, I made it my priority to do amazing in all things science. One…
Slide Two- Aerobic Metabolism Metabolism is vital to the body, as it converts proteins, carbohydrates and fats into energy. Aerobic metabolism requires oxygen to function, unlike anaerobic metabolism (Tang, nd). The oxygen breaks down glucose to release energy in the form of ATP, producing carbon dioxide and water as bi-products. The process of aerobic metabolism includes glycolysis, the citric acid cycle, Krebs cycle and the electron transport chain (Pass My Exams, nd). Slide Three- Pyruvate…
Blood tests show that one or all of the different blood cell types are decreased. One cell type, the lymphocyte may increase and be abnormal in appearance. This can sometimes be confused with certain types of leukemia. If a dog becomes chronically infected, the disease can keep coming back, especially during periods of stress. In some cases, arthritis or a kidney disease called 'glomerluonephritis' may develop. A decrease in the number of platelets in the blood is the most common laboratory…
Histological analysis Tissue harvested from the distal colon (1 cm from the rectum) was cut into segments, fixed with 10% formaldehyde overnight, embedded in paraffin, sectioned at 5 μm, mounted on clean glass slides, and stained with hematoxylin and eosin (H&E) for histological evaluation. Each slide was coded and analyzed in a blinded fashion by two investigators using light microscopy. Each sample was assigned a histological score based on cumulative scores assigned to colon characteristics…
it living or dead. If the observer wishes to see the cells behavior, he would treat it with a dye solution known to not harm the species, and mount it to a microscope slide. If he wished to observe the cell in a static state, he would perform fixation, via heat or otherwise, to kill the cell. Then the cell would be stained and mounted to a slide.) b-The nucleus is an organelle found in eukaryotic cells. Inside its fully-enclosed nuclear membrane, it contains the majority of the cell's genetic…