Phagocytosis: The effects of time and Latrunculin on endocytosis. Introduction Phagocytosis is a process in which cells engulfed foreign materials. Eukaryotic cells can engulf different particles and solutes from their environment using a variety of mechanisms called endocytosis. Some cells can carry out large endocytic processes called phagocytosis and micropinocytosis. These processes can internalize particles (>0.5um) whereas internalization of fluids through micropinocytosis mechanisms…
When conducting the different tests and streaking, aseptic techniques were followed. For every T-streaked that was done, the inoculating loop was first flame for 5 seconds and was allowed to cool for 30 seconds. Afterwards the tube containing the mixed unknown bacteria was then passed over the flame. The inoculating loop was then inserted into the broth and then carefully streak on quadrant one of the plate. Afterwards, the loop was sterilized by the flame and was allow to cool for thirty…
growth surrounding the colony and is confimed by observing dikaryotic filaments, clamp connections, basidia and chain of basidiospore under light microscope as compatible mating type (Wicks BL et al, 1996; Halliday CL et al, 1999) or stereoscope Niko- SZ-PT (Ohkusu M et al, 2002). Slide Mating Methd (Fraser JA et al, 2003):- In this method a sterile glass slide is placed in a sterile petri-dish at an angle of 20o. Then molten V8 juice agar is overlaid to form a thin film on it. Five ml of liquid…
cleanly slice the planarian in half so it could perfectly section of the head and tail section. So a scalpel was used to cut the planarian and it was cut halfway down from the head. It was picked up with an eyedropper, then it was placed on a microscope slide. The slide was cooled so when the planarian would hit the cold, it would not move and it was able to be cut. It was cut right above the stomach and gastrovascular cavity because if that was cut through, the planarian would die and not be…
Introduction The propose of this experiment was to see if we could conducted an experiment that we could elect data and act our hypothesis about sordaria. We hypothesized if tan and black sordaria got placed together to cross over, we could expect out of 50 sordarias that 27 would cross over and 23 would not cross over. Some research has brought up some discussion about how much tan or black sordaria is one the agar square to begin with. (Kitani 1961) If these numbers are more than the other,…
Complete Blood Count (CBC) Jenae Matson WCCC Laurel/Spring 2015 Anatomy and Physiology 2 Matthew Sisak January 23, 2015 Background: Blood is the most important part of your entire body because nothing will function without blood. Blood is there carrier of most important proteins and gasses in your body. Blood has a major part in clotting, protection form unwanted pathogens, passage of oxygen to the body and blood also maintains a balance of fluids, temperature, pH balance and acidic…
membrane was tested using Fluorescent Recovery After Photobleaching (FRAP). We used a Nikon Eclipse 50i upright fluorescent microscope with a Digital Sight DM-2MBW CCD camera to take images of the bilayer after photobleaching. Using these images we were able to determine the diffusion coefficients for the bilayer to be , without cholesterol and , with cholesterol. Using the same microscope and camera alongside an orange Helium-Neon Laser at 594 nm we studied the binding of biotin and anti-biotin…
use a microscope, individuate bacteria on a slide, how it worked a scintigraphy and the radioactive tracer injected into the patients and so on. I loved to go with them at the hospital to look at the laboratory technicians, or to stop in the X-Ray rooms trying to figure out which part of the body there was on the diaphanoscope and what was wrong with it. I always enjoyed so much spending my spare time growing plants, practicing little excavations, preparing slides to look in my microscope,…
gram staining procedure is related to the choice of treatment for the patient. Here is some information about it. Part 1: How Is the Gram Staining Procedure Performed? 1. Prepare the glass microscopic slide. To make sure that your slides are grease/oil-free, wash them using soap and water. Wipe slides with alcohol. This will remove any…
In our study, we had two controls that we placed the Lumbriculus variegatus into. First, we used spring water to get the normal heart rate of the worms. The other control was a caffeine solution that served as our positive control. Our other solutions that we exposed the worms to consisted of increasing concentrations of two different NSAIDs (ibuprofen and aspirin). For each NSAID, we had a solution equivalent to the recommended dosage for a person based on body weight (the proportional dosage…