Microscope slide

INTRODUCTION Being able to identify an unknown specimen in microbiology is vital in the laboratory. The goal of this experiment was to isolate bacteria on a nutrient agar (NA) plate to obtain pure colonies of the unknown specimen. This study was done by applying the methods learned in Professor Sprenkles microbiology lab in determining the unknown bacterial growth present with use of pure culture colony morphology, cell morphology and arrangement, and Gram reaction. MATERIALS AND METHODS The…

cells that we observed were yeast cells. Under the microscope, the only observable differences between the yeast cells and the prokaryotic cells that we have looked at, is that the yeast cells are larger and some cells appear to be budding. When viewed under Phase Contrast, the yeast cells shows different level of contrast on its membrane, possibly due to the organelles within the cells. Other differences that were not observable under the microscope, is that yeast, being a eukaryotic, contains…

sit for a minute, and observed. To begin with this section of the experiment, we acquired 3 leaves from the growing tip of an elodea plant. We then mounted each of the leaves on its own slide and placed a cover glass over the leaf. On the first slide we added distilled water and drew the water across the slide…

three pipets: “surface,” “middle,” and “bottom.” The usage of three different pipets for each layer is to avoid cross continuation. Obtain three different slides and coverslips. Using the surface pipet, take a small sample of pond water form the surface and place one drop on the slide. Place a coverslip on it and then examine under the microscope. Using the objectives, switch to higher magnifications to observe details. Record the observations and using the dichotomous key, identify each type of…

Bunsen burner. Once sterilized, we got a drop of water using the loop and put it on a slide and sterilized the loop once more. Once sterilized, the neck of a culture tube was flamed and bacteria were collected from the agar in the culture tube. The test tube’s neck was flamed once again and the cap was replaced. The water and bacteria was mixed on the slide and allowed to air dry. Once air-dried, we passed the slide over the…

twelve sea urchins prior to the lab: one tested on Friday, four died over the weekend, four were used on Monday, and three on Tuesday. The eggs were collected from the previous class’s one female out the four used. The eggs were observed on a microscope at 10X magnification. We were instructed to gather information on: size, shape, estimate of amount of eggs in solution. We were also going to place the eggs into sumi ink; however, we did not place the eggs within the ink. Individually we…

1. The biggest potential source of contamination of the locations we tested in the Distribution of Microorganisms would have to be the floor of the lab. The reason why this is the biggest potential source of contamination is because multiple people are coming in everyday from the outside world, bringing in microorganisms from the outside into the lab. And although we sanitize the counter and all the spaces that we work around, we don’t take precautions to necessarily clean the floor, making it…

we will plate the C2C12 cells in three different densities on one 6-well plate and two 8-chamber slides. Cells that reached about 80% confluency…

ethanol off with out water not too much. Because the water stop bacteria from decolonization and you want to be able to see the bacteria under the microscope. The last agent you would add was safranin and this is too put back the color to the bacteria. I would then rinse and blot dry on bibulous paper. Then put oil on the slide then observed under the microscope-using eyepiece…

biopsies so that doctors can watch it under a microscope and make a diagnosis. The second step would be the fastening in wax with the result that a smooth and – and this is the most important aspect - a thin cut can be made which is beneficial when you want to see the cell structures properly and very detailed. To see the cells in such a way a colouring must be made. For that, there are some machines which kind of “dip” the finished microscope slides into different concentrations of alcohol,…