Microbiology

Question: How much bacteria will be found in each pedicure spa? Hypothesis: If I swab multiple pedicure spas using a test kit, streak plating, and colony morphology, then I will find the presence of bacteria is unsafe because cleaning practices are not followed properly. Procedures Determine the five pedicure spas in which you will obtain samples from Give each pedicure spa an anonymous name (Ex: Spa A, Spa B, Spa C) Type up an approval letter stating what you will be doing and what it is for…

ABSTRACT: The Microbiology & Immunology Laboratory Unknown Identification Project is designed to test the students’ skills and knowledge of performing biochemical tests learned during the course of the lab. Students receive a numbered tube containing a mixed culture of two unknown organisms and are required to determine the identity of each microorganism by performing a series of biochemical tests of their choice. Unknown test tube #25 was obtained and streaked onto two agar plates to obtain…

Missing Microbes It was an elegantly composed book, loaded with fascinating data about organisms and analyses depicted in an extremely open manner, weaving the individual and expert encounters of its writer, Dr. Martin Blaser, into a provocative analysis on the hazards of improper antimicrobial medication use. He discusses various thoughts of how upset organisms may be influencing cutting edge, endless ailments ("a strange cluster of what I call 'present day plagues': corpulence, youth diabetes,…

inventions have been made possible. Biotechnology is the field emerging with full rush in the employment of microbes in human welfare. Following are the fields discussed in which microbes are being usefully employed. Biotechnology The applications of microbiology on a wider and a commercial level to produce common foods and chemicals and to ease the lives of humans, is known as Biotechnology. It uses microbes or other life forms to produce commercially used products. People have been exploiting…

The abuse of Triclosan, a biocide, also helps to contribute to antibiotic resistance in bacteria through cross resistance development. Triclosan is used in practically every consumer product, ranging from hand soap to plastics and even clothing. Triclosan is incorporated into a variety of products due to its high boiling point of 280 Celsius (Giuliano and Rybak 328). Triclosan is currently classified as an over the counter medication by the Federal Drug and Alcohol administration. This allows…

Arnon, S. S., R. Schechter, T. V. Inglesby, D. A. Henderson, J. G. Bartlett, M. S. Ascher, E. Eit-zen, A. D. Fine, J. Hauer, M. Layton, S. Lillibridge, M. T. Osterholm, T. O. Toole, G. Parker, T. M. Perl, P. K. Russell, D. L. Swerdlow and K. Tonat (2001). Botulinum toxin as a biological weapon: medical and public health management. The Journal of the American Medical Association285: 1059-1070. Bhambhani, A. and C. V. Kumar (2006). Protein/DNA/inorganic materials: DNA binding to layered…

First the unknown tube was isolated. This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate. This was done using the streak plate method, which is described in the lab manual. The bacterium were incubated and grown at 48 hours. Upon observation the isolation plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the…

producing an antibiotic against one or more of the six ESKAPE pathogens (a group of pathogens that are resistant to most if not all current antibiotics). All research protocol was based on the Yale Small World Initiative and the third edition of Microbiology Laboratory Theory & Application. One bacteria from a sample of soil collected (colony #18) was tested for its ability to produce antibiotics against two of the six ESKAPE pathogens. It showed potential against one and further tests were done…

Food is a source of nutrients not only for humans but microorganisms as well. Food safety is a term for all measures and concepts that are intended to ensure that food is safe to eat and cannot cause a health problem or poisoning. The issue of food safety is a universal concern, this is because everyone must to consume food. Bacteria are everywhere, in the air, in the water, in the ground, on our food, on our skin and inside our bodies. Food scares have been numerous in the United States in the…

After gram staining both the gram positive and gram-negative bacteria, the groupings were observed. For the unknown gram-positive bacteria, the cells were arranged in a cocci cluster, while in the gram-negative bacteria, the cells were arranged in a rod cluster. Both the gram negative and gram-positive bacteria exhibit a small yellow colony on the TSA plate. After 48 hours, the MSA plate was observed. Individual isolated colonies were found throughout the plate, with a yellowish color. The…