Methicillin-resistant Staphylococcus aureus

2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…

a post–antimicrobial era in which people may die from the simplest of infections. The CDC estimates that 2 million Americans are infected with drug–resistant bacteria every year, of whom 23,000 do not survive (Antibiotic Resistance Threats, 2013) The greatest threats include Clostridium difficile, vancomycin–resistant Enterococcus, and drug–resistant Streptococcus pneumoniae. The world no longer produces new antimicrobial research at a sustainable rate, thus giving rise to the dominance of…

contributed to this issue, as roughly 80% of antibiotics are used in the livestock industry, often to act as preventive care or to encourage growth (Duckenfield, 2011). Regardless, both of these factors have contributed to the development of antibiotic resistant bacteria, such as MRSA which has negatively impacted our health care system by resulting in the loss of life, decreased quality of patient care, and increased health costs (Gould,…

was investigating the theory that nasal mucus had antibacterial effects when he left a culture plate with Staphylococcus aureus, a deadly bacteria, in his lab while he went on a holiday (Ho). When he returned to his lab, he found that there was a clear halo surrounding growth of a yellow-green mold named Penicillium notatum that had accidentally contaminated the plate (Ho). The Staphylococcus bacteria had grown all over the plate, except the area surrounding the mold growth. Fleming would deduce…

City brings her 6-year-old child to see her primary care physician, complaining that her child has seemed “off” for the past month. The child has been extremely fatigued and taking multiple naps during the day that do not seem to refresh her. She is normally a good sleeper at night and recently has begun to have trouble with multiple awakenings. The child has also complained multiple times of frequent headaches throughout the week. She has also been seen to exhibit some confusion while playing…

bacterial inactivation. Silver Silver nanoparticles have inhibitory activity against Gram positive bacteria and Gram negative bacteria as well. Several experiments conducted against E.coli, B. subtilis, SS. aureus, methicillin-resistant coagulase-negative staphylococci, vancomycin-resistant Enterococcus faecium, ESBL-positive K pneumonia, S. typhi, V cholera to analyse the inhibitory effects of silver nanoparticles.The antibacterial activity are related to the total surface area of the…

Acute Care for the Elderly unit there were several patients on contact precautions due primarily to Methicillin-resistant Staphylococcus aureus (MRSA) or high counts of gram-positive Escherichia coli bacterium (E. coli) in the urine and blood. A good example of best practice that I witnessed was the proper display and implementation of contact precautions around patients with multidrug-resistant organisms and bacteria (MDROs). If a patient is placed on contact precautions on this ACE unit floor…

are resistant to standard prophylactic antimicrobials, as illustrated in Table 5.[37] It was also noted that a 30% decrease in the efficacy of antimicrobial prophylaxis for surgical procedures would result in 120,000 additional SSIs and 6300 infection related deaths per year in the USA alone.[37] Abboud et al reported that in-hospital mortality was 33% for post-surgical mediastinitis (inflammation of the tissues in the mid chest or mediastinum) with the majority a direct result of resistant…

Troublesome Technology Today, most people own cell phones. Those that do own cell phones may know some disadvantages of owning one. What some people do not know is the disadvantages to cell phone usage is not only that they may freeze up or break, but owning a cell phone had far more significant disadvantages such as health problems, automobile wrecks, and effects today’s children. Cell phone ownership is a source of negative health effects. According to Dr. Agarwal, “electromagnetic waves…

Artificial turf has been directly linked to an increase in serious injuries including turf toe, ACL tears, foot lock, turf burns, heat exhaustion, and concussion. Turf toe is a painful hyperextension of the large toe. It is a result of when the cleats of the player’s shoe grabs the artificial turf mesh and causes an overextension of the large toe. Anterior Cruciate Ligament (ACL) tears are one of the foremost common artificial turf injuries. The ACL ligament is located deep within the knee joint…