calyx has the high content of phenolic compounds associating to antioxidant, and have the significant effect on β-carotene bleaching. In freeze-drying, extract in cold water could perform well in some antioxidant property than in hot water, 80 % methanol. We had H. sabdariffa calyx been extracted by cool water and had to freeze-dry, then detected the DPPH scavenging capacity, reducing power, ferrous ion chelating activity,…
the past decade. This can be translated to over 510,000 tons just from last year alone. One of the primary causes of this is because trucks carrying the oil are going to factures 24 hours a day 7 days a week. Not only does oil affect the soil but methanol pollution created can have negative side effects on farm animals and aquatic life. Research has found that animals that drink water that is mixed with fracking wastewater were later found dead an hour later. Pregnant cows that had drank…
INTRODUCTION: Losartan Potassium (LSP)[FIG.1.A], 2-n-butyl-4-chloro-1-[p-(o-1Htetrazol- 5-ylphenyl) benzyl]-imidazole-5 methanol monopotassium salt is a highly selective, orally active, non-peptide angiotensin II receptor antagonist indicated for the treatment of hypertension. It has a more potent active metabolite (II, 2-n-butyl-4-chloro-1-[2- (1H-tetrazol-5 yl) biphenyl- 4-yl) methyl] imidazole-5- carboxyl acid)[1]. The determination of Losartan has been carried out in tablets by HPLC,…
6. SUMMARY AND CONCLUSION 6.1 SUMMARY The current study deals mainly with phytochemical and pharmacological investigation of traditionally used medicinal plant Enicostemma littorale Blume. A perusal of the literature revealed that only fragmental information was available on this plant species regarding cytoprotective potential and immunohistochemistry. In addition the exact molecular mechanism of action of this plant species active compound swertiamarin is unknown and remote. Hence this study…
Chapter 1: Introduction 1.1 Nature of the Dissertation Identification, quantification and analysis Amoxicillin and Flucloxacillin Sugar free antibiotics which are manufactured at Athlone Laboratories by high liquid fluid chromatography (HPLC). The project also involves Method Transfer validation by analysis Amoxicillin Sugar Free for the new project of GSK Mayenne France, this involved Identification, quantification and analysis of carrying Amoxicillin Trihydrate API and Sodium Benzoate in…
In this experiment Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) was performed under reducing conditions to estimate the molecular weight of proteins within a sample. The protein sample was from the previous using anionic exchange chromatography to partially purify N-acetyl--D-hexosaminidase, Lab 3. The molecular weight was determined through the interpolation of a standard curve of proteins with a known molecular weight. The molecular weight of band 1 was determined to…
aureus based on the zone of inhibition which has a diameter range of 7mm to 10mm. In this study, the extract of hexane of S.aromaticum showed positive antibacterial properties against E. coli, and S. aureus for concentrations of 25, 50, 75, 100. The methanol extract showed positive against S. aureus for 75 and 100 mg/ml. The DCM extract showed positive for E. coli, S. aureus, (100 mg/ml). The comparison of the three extracts of clove oil shows that the hexane extract of clove oil has the highest…
spectra of Prazosin + HPMC 7.2.2. Preparation of Calibration curve of prazosin hydrochloride Determination of λ max of prazosin hydrochloride Exhibited peak absorbance at 330nm (λ max) in methanol. Standard solution 50mg of standard drug was accurately weighed and placed in 250ml of volumetric flask and 50 ml methanol was added to it and shakes for few minutes to prepare 1000microgram/ml. Stock solution From this solution the stock solution was prepared by taking 1 ml from standard…
Dex-ibuprofen Dex-ibuprofen (10 mg) was dissolved in 100 mL methanol. From these stock solution aliquots of 0.2,0.4,0.6,0.8,0.10 µ/mlwere withdrawn in 10 mL volumetric flask and diluted up to the volume with methanol and phosphate buffer (ph 7.4) solutions to give concentrations of 2,4,6,8,10 g/mL. Absorption of each solution as calculated at 227.2 nm ,223nm using Elico SL 210 UV-1700 UV Visible double beam spectrophotometer and methanol as well as phosphate buffer (ph 7.4) as a reference…
compares the heat capacities of water and two other solvents. Compare the specific heat capacity of water to other substances (e.g ethanol) providing an example of why water is used in industry based on this property. Results & calculations 힓H = mC힓T Methanol CH3OH Propenol C3H8O Ethanol C2H5OH m = 3.6g m= 3.4g m=8.3g…