Methanol

Manufacturing Company. Methyl paraben is methyl-4-hydroxybenzoate, prepared by the esterification of p-hydroxybenzoic acid with methanol. It occurs as colorless crystals or a white crystalline powder. It is odorless or almost odorless and has a slight burning taste (Rowe et al., 2009). Methyl paraben is very slightly soluble in water, freely soluble in alcohol and in methanol (British Pharmacopoeia 2009). It is widely used as an antimicrobial preservative in cosmetics, food products, and…

I. Abstract The experiment was about using a method of recrystallization to reform benzoic acid and unknown compound, and identifying that compound. The main principle is the polarity and solubility of compound. The compound was dissolved completely with distilled water in hot temperature and reformed back in cold environment. After the experiment, there were up to more than 50% of the compound being recrystallized and the unknown compound had the melting point at 165.2oC. The experiment…

The product air-dried for about one week. After this time, the 9-fluorenol was recrystallized by a mixed solvent recrystallization4 using methanol and water over the steam table. The solution was cooled to room temperature and put in an ice-water bath for ten minutes. The crystals were collected and placed in the oven to dry for fifteen minutes. The product was weighed to calculate percent…

diesel oil. 5.2 MATERIAL AND METHODS The Mahua oil methyl ester was prepared by using three stage transesterification processes because their FFA level is more than 1%. In the first step, esterification reaction was carried out by adding 15 ml methanol to 100 ml Mahua oil and 0.5ml H2SO4. This reaction period was carried at temperature range between 550c to 600 c with reaction…

wheat kernels in 150 μm sieve; Wwheat in 75μm sieve = weight of wheat kernels in 75 189 μm sieve; Wpan = 190 weight of empty pan; and Wmaize meal = weight of maize flour. 191 Determination of Crude Protein 192 The crude protein content of the roasted maize was determined in duplicate by Dumas combustion 193 method as described in AACCI Approved Method 46–30.01 (AACC, 1999) using a Leco, model 194 FP-528 (Leco Africa, Kempton Park, South Africa). Calibration of the instrument was done using 195…

in the solution, which produced a slight yellow coloring. Water was added to the solution before pouring onto a Hirsch funnel for separation. The crystals were washed with methanol, and removed from the filter to dissolve in methanol and recrystallization. The crystals had a white, clear appearance. These were added to methanol and heated for recrystallization. It was noticed that small black particles had appeared in the solution. The filtration step was repeated before measuring the mass and…

(F1), followed by ethyl acetate: petroleum ether (F2, 20 : 80), ethyl acetate: petroleum ether (F3, 40 : 60), ethyl acetate: petroleum ether (F4, 60 : 40), ethyl acetate: petroleum ether (F5, 80 : 20), 100% ethyl acetate (F6), methanol: ethyl acetate (F7, 20 : 80) and methanol: ethyl acetate (F8, 40 : 60). Sl No Fractions Yield (g %) F1 100% petroleum ether 1.2% F2 ethyl acetate: petroleum ether (20 : 80) 4.9% F3 ethyl acetate: petroleum ether (40 : 60) 19.4% F4…

Test tube # Solvent Observations 1 Cyclohexane The liquid is clear 2 Ethanol The solution is a tinted yellow liquid at room temperature 3 Methanol Slight brown tint, unknown mostly dissolved 4 Hexanes The liquid is a very clear color Figure 1: Table of possible solvents and their results 1. The solvent that seemed most appropriate to use was Methanol, and after confirming that it recrystallized in an ice bath, we decided that it was the solvent that we were going to use 2. We used 1.358 grams…

REACTION RCOCH3 + 3I2 + 4NaOH ͢ RCOOˉNa⁺ + 3NaI + 3H20 + CHI3(s) This test is positive only if a yellow precipitate appears. Pale yellow precipitate indicates the presence of methyl ketone but the other ketone will discharge colour. Unknown B, methanol, cyclohexane and acetaldehyde are not ketones since they did not produce a yellow precipitate. They are aldehydes and alcohol. The unknowns were expected to have parallel similarities to the other carbonyl compounds. These compounds were…

2.5.5 Western blotting Western blotting was done to separate and identify the size of proteins that react with a specific antibody. After the proteins was transferred onto the PVDF membrane, it was incubated in 5% blocking solution for 1 h at room temperature with constant swirling with belly dancer (Storall Life Science). The blocking of the membrane is to prevent the nonspecific binding of the detection antibodies during next step. After that, washing of membrane was done by using 0.05% TBST…