Kanamycin sulphate and rifampicin were purchased from Hangzhou Dayangchem Co., Ltd., Zhejiang, China. Acetonitrile and ethanol (High performance liquid chromatography, HPLC, grade) were purchased from Merck, Germany. Sodium dihydrogen phosphate, phenylisocyanate, triethylamine, orthophosphoric acid (analytical reagent grade) and silicone oil (viscosity 10 cSt) were purchased from Sigma–Aldrich, St. Louis, USA. Size 3 hard gelatin capsules were kindly donated by Capsugel Co., Ltd., Tokyo, Japan.…
Since the industrial revolution fuels have been used for power. Fossil fuels are organic matter made from remains of flora and fauna which has been subjected to immense pressure and heat deep within the earth over millions of years. Now fuels such as petroleum are used to power vehicles such as cars airplanes and ships (Mitshibishi Heavy Industries, 2015). Fuels are products that are burned to release energy in the form of heat or power, fuels are made out of coal, oil, natural gas, and wood and…
These are the household items that anyone can purchase. Industrial chemicals such as chloroform, toluene, benzene, methanol, coolants, paints, glues, and gases. These are the most dangerous, even to a first-time user. The general method of consuming solvents is called “huffing”. Initially, blood levels peak, dizziness, disorientation, light-headedness, muscular incoordination…
Transfer all medium from each well to 1.5 ml tubes. Centrifuge at high speed 10000g for 1 min in Eppendorf tube. Transfer supernatant to 1.5 ml tube and snap frozen in liquid nitrogen then kept at -80 °C until hormonal analysis. Trypsinization were used for harvesting live cell, 500 µl of pre-warmed trypsin-EDTA were added to each well and incubated at 37.5 °C for 5 min. Then dislodged cells from well by pipetting and to stop trypsin effect, 100 µl of heated inactivated fatal calve serum (HI…
There have also been studies that suggest that chemicals used during fracking, such as methanol, formaldehyde, sodium hydroxide etc., are carcinogens 2. When fracking occurs in a specific site, the chance for contamination is low but as the number of sites increase, the chance for contamination increases 4. It is suggested that advanced testing…
applications in optoelectronics, sensors, transducers and bio-medical sciences [2]. the control over ZnO’s rich defect chemistry which is important in controlling properties is the reason for having its vast range of applications. ZnO usage in low pressure methanol synthesis as a component of composite catalysts as it prolongs the catalyst life [3]. ZnO after reduction leads to the formation of oxygen vacancies this in turn helps the catalytic activity by acting as an active…
Magnesium (1.5 g) was stirred vigorously, to combat oxidation and pulverize the by-product magnesium sulfate, in a 250 mL round bottom flask for 5 to 10 minutes, then fit with reflux apparatus – including CaCl2 Drying Tube, 125 mL Dropping funnel, Claisen adapter, and thermometer adapter – and add anhydrous ethyl ether (40mL) through the dropping funnel. Bromobenzene (3mL) and Anhydrous Ethyl Ether (15mL) was added to the reaction flask through the dropping funnel dropwise and in halves. Reflux…
It is typically assumed that all tequilas are the same 100 % agave drinks although remarkable part of them are actually mixed spirits. There have been a few attempts to study the composition of tequila samples using gas chromatography and headspace solid-phase microextration but these techniques have proved to be too costly for routine analysis. Lachenmeier and colleagues observed that the authentic tequila research required more advanced and affordable method for separating the 100 % agave…
1. Duke Energy jBy merging with Progress Energy in 2012, Duke Energy Corporation (NYSE: DUK) became the largest electric utility in the United States. Headquartered in Charlotte, North Carolina, its regulated utilities business unit serves 7.3 million retail electric customers in six states in the Southeast and Midwest regions, boasting approximately 57,500 MW of electric generating capacity. Almost all of its electricity generation comes from coal, natural gas and oil. As of 2015, it has 28,000…
problems, from wood-chips digestion, spent liquor evaporation and bleaching as volatile organic carbons (VOCs), and from combustion process as nitrogen oxidies (NOx) and SOx. VOCs also include ketone, alcohol and solvents such as carbon disulfide methanol, acetone and chloroform…