Medium of exchange

Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…

Objective I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient…

(the tool): A wire made into a loop to assist in picking up inoculum from a culture of microorganisms. The loop is also used in the process of cultivating microbes by transferring inoculum for streaking. 3. Agar vs Blood Agar: a. Agar: a growth medium made with agar and other nutrients. The agar allows microorganisms such as fungi and bacteria to be cultured. b. Blood Agar: Blood agar is used for the purpose of cultivation and isolation of various strains of pathogenic bacteria. 4.…

Looking up at the night sky, little glowing dots are scattered across the night sky. They are known as stars. There are several billion stars in our galaxy, but only a few are seen with the naked eye. Stars are by definition, large exploding balls of gas made up of mostly hydrogen and helium. The two most unstable elements on the periodic table. The largest star in our galaxy is the sun. It’s the largest star and one of the few stars that are going through a star wide nuclear reaction. The…

Introduction : Enterotube II is used to identify multiple tests in one tube. twelve different conventional media plus a self-contained inoculating wire. The twelve test are for glucose and gas, then lysine, ornithine, H2S and indole, adonitol, lactose, arabinose, sorbitol, dulcitol, phenylalanine (amino acid), urea, and citrate (No VP test require). These medias performs 15 different biochemical tests that will determine the genus and the species of gram negative bacteria. This test will help…

Ever wanted to know more about nebulae or galaxies? A nebula is a cloud of interstellar dust, stars, and gas (usually hydrogen and helium). A nebula is less dense than a vacuum and mass less than a few kilograms although it is usually the size of the earth or bigger. A galaxy is gravitationally pulled system of stars, stellar remnants, interstellar gas, dust and dark matter. Galaxies range in size in lightyears. Galaxies, they are what holds our solar system and many, many more. We may not be…

The purpose of this experiment was to swab a chosen object, isolate a microorganism, and identify the microorganism through a series of morphological, physical, and biochemical tests. One chose to swab David Phelps’ pastor dreamer, which is one of the larger statues on The University of Oklahoma’s campus. The nose of this statue was swabbed and is where one’s environmental isolate originated. Through morphological, physiological, and biochemical experiments, one was able to gather results that…

Materials and Methods: To obtain the live bacterial cultures for this experiment 3 petri dishes were, using Aseptic techniques, inoculated with bacteria. In each petri dish was a sterile agar, rich with nitrogen and glucose to ensure the growth of any bacteria placed on its surface. Each petri dish was inoculated from different bacterial source which were, each group members palm (the petri dish was divided into quarter each member inoculated only their section), the touch screen of a cellphone…

The purpose of the unknown experiment is to understand and identify a gram positive and a gram negative. Bacteria can be very helpful in clinical uses and many pharmaceutical drugs are based on the organism. Knowing how a bacterium works and how it is structured can be crucial to determine how it affects each individual. Several biochemical tests were performing to separate and determine the identities of the microorganisms. Broth tube number 31 containing unknown microorganisms. One of the…

I look up to the sky and see millions of stars, and I wonder to myself. What makes them light up so bright, and what makes them a star? What does it take to create a star; they just don’t pop out and shine bright. I want to know the process, and how long it takes for a star to be born. I am also curious to know if the star can die. I plan on finding the answer. I believe that they are created by gases and that they die when they use up all of their gases. Stars have had an impact in our…