Medium of exchange

Objectives: The purpose of this project is to identify two separate microbial organisms from a given list, which are to be inoculated from a mixed broth culture. Multiple tests will be performed in order to eliminate all organisms from the list that do not provide significant results for the identification of the unknown specimens. Journal Entries: April 17, 2018. Today we began the unknown project. I obtained the unknown broth tube #15. I used two Tryptic Soy Agar (TSA) plates in order to…

The purpose of the unknown experiment is to understand and identify a gram positive and a gram negative. Bacteria can be very helpful in clinical uses and many pharmaceutical drugs are based on the organism. Knowing how a bacterium works and how it is structured can be crucial to determine how it affects each individual. Several biochemical tests were performing to separate and determine the identities of the microorganisms. Broth tube number 31 containing unknown microorganisms. One of the…

I look up to the sky and see millions of stars, and I wonder to myself. What makes them light up so bright, and what makes them a star? What does it take to create a star; they just don’t pop out and shine bright. I want to know the process, and how long it takes for a star to be born. I am also curious to know if the star can die. I plan on finding the answer. I believe that they are created by gases and that they die when they use up all of their gases. Stars have had an impact in our…

As people decided to sending human into deep space to explore unknown feature of the universe, the environment outside the earth is a big uncertainty with factors that we can’t fully understand yet. Besides the cost and the time we spend during long space travel, stay in the cold, vacuum universe would let astronauts experience different kind of radiation. These radiation may be some ionized particles as electromagnetic waves traveling with high speed and energy. They could come from nearby…

Journal 1 After the first two weeks of lecture in Cinema Appreciation I have learned a great deal more about what goes into making a film. I was under the impression that there was one magical camera that could change settings to capture the diverse range of images instantaneously. Active viewing was something, I thought I did, but it wasn’t until I took a step back and saw the variety of shots and how each one of them is put together that I realized that there is more then one way to watch a…

Transformation of pGlO plasmid to an E.coli bacteria cell We pipetted 250 ul of CaCl2 transformation solution to into two separate tubes. Next, we used a sterile loop to transfer two to four larger bacteria colonies from an E.coli plate into each of the two tubes. The bacteria was observed under UV light to make initial observations. We pipetted 10 ul of pGLO plasmid (0.08 ug/ul) into one of the tubes. Both tubes were incubated on ice for ten minutes. After ten minutes of incubation, the tubes…

Discussion: Each test is a pertinent contribution to drawing an educated conclusion. After performing the gram staining and observing which unknown culture was gram positive, a catalase test was performed. The catalase test allows lab workers to differentiate Staphylococci and Streptococci genus. The test was performed only on the gram-positive culture. Bacteria that respire by the use of oxygen, otherwise known as aerobic respiration, produce enzyme or “catalase”. This protects the bacteria…

6. Three Nutrient Agar Plate (NAP) were contaminated as follow to illustrate that there are microorganism all around us and to demonstrate the necessity for proper aseptic technique. a) The lid of the first agar plate was removed and the exposed agar was placed in the building on the laboratory rack for 3 hours. The lid was replaced and labelled as “air”. It was then incubated at room temperature. b) A second Petri plate was divided in half using a marker. Two cotton sterile swab were moisten in…

For this experiment, unknown sample #12 was used. The first step in identifying any unknown bacterium is to grow a culture on a nutrient agar plate. This bacterium was also plated on a MAC plate, which is both selective for gram-negative bacteria and differential as well. On the NA plate, bright red colonies were present; on the MAC plate there was bacterial growth as well, but no color change was observed. This lack of color change on the MAC plate indicated that the bacteria did not ferment…

Mtb is classified as a Gram-positive bacteria, and is one of the few bacteria that can be cultured in the laboratory5. Its cell surface is partly composed of various virulence lipids, such as phthiocerol dimycocerosates (PDIMs) and phenolic glycolipids (PGLs). and mycolic acid3. Mtb, like many other bacteria, is capable of sensing and responding to their surrounding environment. It does so via the use of two-component systems, one of which is called PhoPR two-component system6. In order for Mtb…