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    454 Sequencing

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    In 2005, new sequence technique, which known as next-generation or second generation sequencing, was emerged after three decades of Sanger sequencing dominance. The new technique, 454 pyrosequencing, has completely new mechanism compared with the previous one, where this technique depended on series of enzymatic steps that including four enzymes polymerase, ATP sulfurylase, luciferase and apyrase under pyrosequencing mechanism. The name 454 pyrosequencing derived from pyrophosphate that realised…

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    Speaker try to organize in the first main point in the speech speaker demonstrate the need for a new policy by showing the extent and seriousness of the problem and followed in the second main point when the speaker explain their plan for solving those problem and show it practically b. Problem Cause Solution Order The second pattern can be used is problem cause solution order,this is a variation from problem solution order before because in the first main point in the speech speaker…

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    EVALUATION Harvey’s House Scene This is the first scene in our sequence which happens in the climactic point of the film. We wanted to build up a lot of tension in this scene before the chase scene so would need to include a variety of slow moving camera shots and close ups. The emptiness of the house followed by reaction shots clearly show how tension is being raised; the audience not knowing quite is about to happen in the coming moments. The lighting in these scenes are a perfect example of…

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    Pursuit of Happiness “Run From Taxi Scene” In the film The Pursuit of happiness directed by Gabriele Muccino, during a sequence of events in which the protagonist, Christopher, does not have the money to pay the taxi for a ride thus he has to run from the taxi driver who proceeds to chase him. This sequence uses several film techniques to add to the effect of this moment with the intention of showing what challenges Gardner will have to face in order to…

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    of Sherlock Holmes, every case develops through the eyes of Dr. Watson. Furthermore, all information about Holmes' solitary investigations is only made known to the reader when the detective shares it with his partner. In contrast, there are many sequences in the movie that show Sherlock's actions without them ever being disclosed to the doctor. Additionally, many scenes do not even show Holmes or Watson, instead focusing on other characters and events, such as Lord Blackwood's crimes and Irene…

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    is an important protein in human function. LDL receptor is the main protein that binds and carries cholesterol to maintain homeostasis in mammalian cells. In human plasma, it is also the most abundant cholesterol-carrying lipoprotein (Goldstein and Brown, 1987). It plays a major role in the development of atherosclerosis, a chronic disease caused by the accumulation of LDL-cholesterol in the blood. The LDL receptor is also the main protein that is defective in individuals with familial…

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    a. Our group used Monroe’s Motivated Sequence and problem-solution pattern as our two main ways of organizing our information and our speech. We used problem-solution because we talked about how yoga can be beneficial for your physical health and your mental health. One of the problems that yoga can reduce is how much stress college students have. By doing yoga, students can become more relaxed and relieve some of the stress that they have because of college. We also talked about how yoga can…

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    identify and match all upper and lower case letter with prompting, and a 75% accuracy when asked to retell the main ideas or important facts from a read aloud. Based on these scores, I plan to raise the accuracy percentage goal for identifying and sorting colors, maintain the percentage accuracy goal for identifying upper and lower case letters, and raise the accuracy percentage goal for retelling main ideas or important facts. In terms of learning goals, the focus student has a bilateral…

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    probe designing. Objective: The main goal of this original article was to design 15 microarray probes as the best candidates. Method: For performing this research, the complete genomes of F.tularensis subsp. tularensis…

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    Assembly Database Paper

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    DATABASES 1. Assembly Assembly database is needed to provide a systematic organization of genome. To accomplish that, the genome is fragmented into small pieces and is sequenced for many times to try to recreate the set of chromosome sequences. This database provides a unique tracker in form of accession so that users can identify changes to an assembly in precise locations whenever needed. There are two different types of assembly updates which are major release and minor release. 2.…

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