A clean flame test wire was inserted into 0.2 M NaCl and also placed on the flame reputedly. A bright yellow persistent flame was a positive test of sodium being presented, then repeat the same instructions for the unknown ion. After the flame test wire was clean 0.2M KCl was dipped into the flame resulted a lavender color after several trials as well as the unknown solution. 10 drops of 0.1M Ca(NO3)2 in a test tube and 2-3 drops of 1M Na2C2O4, a white precipitate of calcium was present. The…
in a mixture. Materials: Test Solutions and Substances 6 M hydrochloric acid, HCl Sodium bismuthate, NaBiO3 (s) 6 M nitric acid, HNO3 0.1 M potassium thiocyanate, KSCN 6 M ammonia/ammonium hydroxide, NH3 3% hydrogen peroxide, H2O2 6 M sulfuric acid, H2SO4 Sodium bismuthate, NaBiO3 (s) 6 M sodium hydroxide, NaOH Cation Solutions 0.2 M silver nitrate, AgNO3 0.5 M lead (II) nitrate, Pb(NO3)2 0.2 M mercury (I) nitrate, Hg2(NO3)2 0.2 M iron (III) nitrate, Fe(NO3)3 0.2 M copper (II) nitrate,…
Purpose: To find the pH of different household items and to see what substances are acids or bases. To see how different substances neutralize each other. Hypothesis: I hypothesize that cleaning products will be bases and foods and substances we ingest will be acids. Materials: • Oil • Finesse Shampoo • Anti Freeze • Drain Opener • Hand Soap • Vinegar • Oxy clean • Pine-Sol • Hydrogen Peroxide • Apple Juice • Ammonia Procedure: 1. All materials were gathered, and safety goggles were put on.…
cross-linker and α,α′-Azoisobutyronitrile (AIBN) were purchased from Merck (Hohenbrunn, Germany). MAA was distilled under vacuum to remove the inhibitor prior to polymerization. To clean the inhibitor from EDMA, it was washed with 10% aqueous sodium hydroxide three times, followed by washing with pure water, and then dried over anhydrous sodium sulfate. Ultra pure water used for HPLC analysis and MISPE process were obtained from a Direct.Q3 Water Purification System (Millipore Corporation, USA).…
c, phenolphthalein, methyl orange, deionized water and the following chemicals: sodium hydroxide, potassium hydrogen phthalate, hydrochloric acid and sodium bicarbonate. When it comes to these chemicals, make sure to wear the general protective personal equipment and handle with caution because these chemicals can burn. (MSDS Solution Center) For week one, the students first had to prepare the sodium hydroxide and the hydrochloric acid. Both of these were done by taking a graduated cylinder and…
Benzimidazole and its derivatives were synthesized according to the reported literature (Wagner EC et al., 1943). The compounds are benzimidazole ;1-(4-((1H-benzimidazol-1-yl) methylamino) phenyl) ethanone; 2-butyl-1H-benzimidazole; 2-methyl-1H-benzimidazole. The stock solutions are prepared by dissolving the compound in DMSO. Antimicrobial activity was tested by the disc-diffusion method under standard conditions using Mueller-Hinton Agar (MHA) and the antibiotics used as listed below, Table…
The purpose of these calculations is to identify the limiting reagent and calculate the percent yield of the esterification reaction used to produce aspirin. This reaction was catalyzed by concentrated H2SO4, but since it is not consumed during the reaction, it does not affect the stoichiometric calculations. In order to determine the limiting reagent, the moles of reactants were calculated (Part A). Part B of the calculations illustrates the amount of aspirin produced by each reactant based on…
Methods: To begin, our group acquired three premade vials of .1ml mammal blood with coagulant. We then labeled the vials numbers one, two, and three. In vial number one we added 1 ml of Stock 300 mM NaCl, and no distilled water. This resulted in a NaCl concentration of 1.8 percent. In the second vial we added .5 ml of Stock 300 mM of NaCl, and .5 ml of distilled water. This resulted in a NaCl concentration of .9 percent. In the third vial we did not add any Stock 300 mM of NaCl, and 1 ml…
A range of concentrations (1nM to 30μM) of acetylcholine (Ach) and 5-hydroxytryptamine (5-HT) was administered to organ bath containing rat ileum. Ach and 5-HT induced contractions from 3nM (Fig. 1) and 10nM (Fig. 2) onwards respectively, both generating a shallow slope at the beginning of the concentration-response curve. EC50 of Ach is 7.47 x 10-7M whereas EC50 of 5-HT is 7.97 x 10-7M. The contraction generated by Ach and 5-HT was expressed in % Emax. As shown in Fig. 1 and 2, % Emax…
Malachite Green Assay: The reaction that AGPase catalyzes produces PPi. The resulting PPi that is produced is hydrolyzed by an inorganic pyrophosphatase (Sigma Aldrich) to generate phosphate (Pi). Each reaction for the assay was conducted on a 96 well plate and the reaction mixture was 50µL and contained Pre-Mix which contained 50mM HEPPS, pH=8.0 (MP Biomedicals), 10mM MgCl2, 1.5mM FBP (Sigma Aldrich), and 0.2mg/mL Bovine Serum Albumin (Sigma Aldrich), and 1.5mU/µL yeast inorganic…