Lysis

degrees Celsius where it was able to grow overnight. After it was able to grow the plasmid DNA was isolated using Quick Lyse Minirep. The mixture was then mixed in order to resuspend the cells that had settled on the bottom and pipeted into a Quick Lyse Lysis. This tube was then Cetrifuged at 13,000 rpm and washed with a buffer. After being washed it was eluted with another buffer and set aside for the next part of the…

Introduction The cultivation and enumeration of a bacteriophage is a vital component of microbiology. Bacteriophages are used for a number of scientific applications, including the decontamination of food products, human therapy, and much more, thus it is critical to determine the concentration of the bacteriophage in a solution. Doing so allows scientists to determine bacteriophage potency, side effects, and minimal effective doses for clinical applications (Andersen et al., 2011). This…

To answer the question, it is first necessary to understand what a polysaccharide is and what hydrolysis is. The four "building blocks of life" is a commonly used phrase that refers to four kinds of small molecules that are essential in the creation and formation of larger macromolecules that exist in every living organism. These four molecules consist of simple sugars, fatty acids, amino acids, and nucleotides. "Poly-" means "many", and "saccharide" means "sugar," so as the name suggests, a…

The modern era advanced the biological science industry with the introduction of biotechnology. The biotech industry allowed the use of technological advancements that assist the use of research. Biotech has also allowed scientist to look at biological topics from a microscopic scale to a nanoscopic scale. One of the biggest innovations is the ability to research single cells. The research of single cells is a topic that is able to be assisted by Fluidgm technology, a biotech company that…

diabetes, we can find cases in which the insulin is absent, little insulin or the function of insulin is impaired or not functioning. The insulin is produced and secreted by cells in the pancreas. Insulin also stimulates gluconeogenesis is that inhibits lysis regulates glycogen and protein synthesis. Pancreatic glucagon stimulates the breakdown of glycogen and gluconeogenesis. Cortisol promotes gluconeogenesis and Epinephrine is a neurotransmitter that increases the breakdown of glycogen. The…

Introduction Parkinson’s disease (PD) is a neurodegenerative disease, with prominent motor symptoms (i.e. rigidity, tremors and bradykinesia) [1, 2] and non-motor symptoms [3, 4] (i.e. sleep disorders, constipation, cardiac arrhythmias and cognitive deficits), ultimately leading to death. There are two defining features of PD, degeneration of dopaminergic neurons in the substantia nigra and an abundant amount of -synuclein protein in the brain creating Lewy bodies [5]. When -synuclein misfolds…

FLUCYTOSINE Also known as 5-flurocyrosine or 5-FC. It’s an antifungal drug that is usually formulated with amphotericin for wide range of medical uses, it can be used by mouth for treatment of infections that could be caused by Cryptococcus neoformans or perhaps candida. It could also be used in the treatment of chromblastomycosis (chronic fungal infection of subcutaneous tissue or skin). Because of its weak antifungal action it can’t be used alone for treatment of serious, life threatening…

Vocabulary 1. Inoculum: a material used for inoculation. 2. Loop (the tool): A wire made into a loop to assist in picking up inoculum from a culture of microorganisms. The loop is also used in the process of cultivating microbes by transferring inoculum for streaking. 3. Agar vs Blood Agar: a. Agar: a growth medium made with agar and other nutrients. The agar allows microorganisms such as fungi and bacteria to be cultured. b. Blood Agar: Blood agar is used for the purpose of cultivation and…

Leukocidins are S.aureus toxins consist of two separate monomeric subunits which have the ability to bind to leukocyte membrane forming β barrel pores that extend through the phospholipid bilayer leading to lysis of leukocyte (fig. 1). There are six known leucocidins (lukED, lukSF, HlgAB, HlgCB, LukAB/HG and LukMFʹ). All S.aureus strains can produce three types of leukocidins at least (LukAB/HG, HlgAB, and HlgCB). Also, all leucocidins can infect human except LukMFʹ which can detected on…

but include men and infants. This disease is the result of a bacterium that is transmitted through sexual activity. This can be any activity that infects the oral, vaginal or anal mucosa. This introduction of bacteria results in cell destruction by lysis. The average course of the cycle is 48 to 72 hours. In women, the disease spreads in an ascending fashion. It starts with the cervix, then to the uterus and finally fallopian tubes. Once the disease is past the cervix it is usually classified…