Lysis

Discussion: The isolate studied was tested for innate antibiotic function on the following media: AC, LB, and 10% TSA. The ESKAPE relative strain that presented a response to the isolate was B.subtilis. In all cases except for that of LB, the isolate showed antibiotic qualities towards B.subtilis by presenting a zone of clearance on AC and 10% TSA media. This behavior is shown in Figures 4, 5, and 6.In the case with LB, B.subtilis inhibited the growth of the isolate as depicted by the empty…

Sexual differentiation in monomorphic avian species Madeleine Evans ID: 213023921, Deakin University Waurn Ponds, Course S323 Biomedical Science, Subject SLE254 Genetic Introduction Sexual differentiation is one of the first biological processes to occur during the development of a foetus. There are a variety of methods for sex determination amongst vertebrates including analysis of primary and secondary differentiations. Primary differentiation refers to the presence of gonads, while secondary…

1. Describe 3 targets of antibiotics, why each target is unique to the particular bacteria, and under what circumstance the bacteria could be resistant to that particular drug. (5 pts) As the name implies 50s subunit protein synthesis inhibitors such as erythromycin inhibit protein synthesis by binding to the 50S subunit of the 23S rRNA molecule of the bacterial ribosome. This ultimately acts to block the exit of the growing peptide chain. It should be noted that gram+ bacteria more…

NAME UKAH R.C MATRIC NUMBER NSC/2014/887 COURSE CODE PCL 304 ASSIGNMENT : QUESTION NUMBER 1: LIST THE PRINCIPLES OF CHEMOTHERAPEAUTIC RESISTANCE Introduction: PRINCIPLE : Principle is an essential factors or elements that may have impact on the survival mechanism, which may either be from environment or the person taking the drugs or factor participating may engineer drug resistance. RESISTANCE: It is reduction in…

Sejaphala M.D. Student No. 62399543 Unique Number: 770819 Introductory Microbiology MIB2601 Assignment 02 Due Date: 13 April 2018 QUESTION 1 1. Bacteria is stained using a primary stain called crystal violet and then fixed with a mordant which in most cases is iodine. While some bacteria have the ability to retain the primary stain, others discolor when exposed to the acetone and alcohol (95%). Hence, the Gram stain divides bacteria into two groups- these bacteria are grouped…

Below I am discussing, at length why I believe I should receive the Research Plus Award, addressing each point of the Researcher Excellence Framework, giving evidence how the past two and a half years have contributed to my personal and my career development. 1. Knowledge and Intellectual abilities a. Knowledge base Throughout my PhD I have been able to attend a number of courses organised by the QUB postgraduate centre, which allowed me to further my knowledge and gain valuable skills within my…

maximum speed. Once centrifugation is completed the supernatant was removed and 250 microliters of re-suspension (R3) was added to the remaining bacterial pellet. This was then re-suspended using a pipet, until homogeneous. Then 250 microliters of lysis solution was added. This solution contains SDS detergent, which dissolves the cell membrane and denatures proteins. Then the tube was gently mixed by inverting four to six times. Following the inversion, 350 microliters of precipitation buffer…

In a cell, substance can move in and out without expanding energy; which is called passive transport. Think about molecules and ions move in motion in a solution. When a random motion cause a net movement of a substance of high concentration to low concentration is called diffusion. That is where the molecules spread out evenly throughout the area. When you have a difference in the concentration in a distance is called a concentration gradient. Random movement of molecules are called Thermal…

INTRODUCTION Lovastatin is a statin drug that decreases the total cholesterol level in patients diagnosed with hypercholesterolemia. The drug aims to prevent serious cardiovascular diseases such as heart attack due to blockage of arteries. Lovastatin acts as a competitive inhibitor for the HMG-CoA reductase enzyme in catalyzing the conversion of HMG-CoA to mevalonate, (Alberts, 1998). The drug is also hydrolyzes in vivo to active form β-hydroxyl acid, (Katz, 2005) and was recently researched on…

It is important to make an accurate diagnosis in this case as the risk of severe illness and treatment varies between an uncomplicated and a complicated UTI.1 The absent of classical presentation of loin pain and rigors would make acute pyelonephritis very unlikely. She was also haemodynamically stable on examination and showed no signs of urosepsis. Hence, based on this patient’s complaint of dysuria, frequency and suprapubic pain accompanied by urinalysis findings of leucocytes and nitrites;…