Listeria monocytogenes

2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…

INTRODUCTION : Ghee is a class of clarified butter that originated in South Asia and is commonly used in South Asian (Indian, Bangladeshi, Nepali, Sri Lankan, and Pakistani) cuisine and ritual. Ghee is a type of clarified butter that is prepared by boiling butter and removing the residue. Spices can be added for flavor. Ghee has a long shelf-life and needs no refrigeration if kept in an airtight container to prevent oxidation. The texture, color and taste of ghee depends on the…

Enterobacter spp. Enterobacter spp are part of the enterobacteriaceae family. They are facultative anaerobic gram negative bacilli. Their means of motility is by means of petrichous flagella. They produce acid upon glucose fermentation and are methyl red negative, voges-proskauer positive. Optimal growth temperature is 30*C .80% are encapsulated. E. spp have been associated with nosocomial outbreak and are opportunistic pathogens. They can cause numerous infections such as cerebral abscess,…

“The brain is important in the human body because it allows a person to think, feel and store memories, and it controls and coordinates the body’s actions and reactions,” states the Atlanta Brain and Spine Care. Because of the great importance of the brain, our body has many different parts and way of working to protect it against any harm or damage. The protective membranes that surround the brain and spinal cord are called the meninges. The meninges consist of three layers, the dura mater,…

Francisella tularensis is an important microbial agent which causes the severe infection of tularemia. The intracellular life cycle of this bacterium is supported by a vast range of protective guards such as enzymes. Although several enzymes are recognized in different subspecies of the bacterium, acid phosphatase and superoxide dismutase are seen in all bacterial subspecies. On the other hand, the most important enzymatic system relating to host cells is NADPH oxidase. For this reason, the…

Term Paper: The Effects of Foodborne Illness of Escherichia coli in Leafy Greens Introduction Food is a necessary and many times pleasurable part of everyday life. Bacteria are also a necessary part of everyday life, but not often thought of as pleasurable. Humans are made up of approximately 103 cells, and harbor about 104 bacteria cells (Davis 1996). It is shocking that the human body hosts more bacteria cells than its own body composition. Fortunately, most of these bacteria that live on…

INTRODUCTION Two significantly important highly pathogenic infection diseases namely Legionellosis and Influenza remains even today a threat to global health. They can cause severe community-acquired pneumonia with respiratory failure but they can also generate hospital-acquired infections.1 Moreover Legionella infection could attribute to influenza infection. The cause of influenza was definitively resolved back in 1930s with the isolation of swine influenza; a virus which when administered…

A business owner must decide on a suitable location and advertise for staff with the correct training for the position to ensure they will do their job correctly and efficiently. When starting a food business, you should carry out a risk assessment and what to include. They must use Hazard Analysis and Critical Control Point (HACCP) procedures or a HACCP-based Food Safety Management System. Kitchen workspaces should be designed to create workflow and its crucial in preventing…