Liquid-liquid extraction

In this experiment, solid-liquid extraction was used to separate trimyristin from the other components in nutmeg. The result was then tested for purity using melting point analysis. This extraction technique uses differing solubilities of components in a chosen solvent system, in this case diethyl ether, as a means of separation. Utilizing this technique, of the original 3.8146 grams of nutmeg, 0.1905 grams of trimyristin were recovered, yielding a 4.99 % composition. This methodology can…

This makes the liquid boil at a lower temperature. Figure 5 DIstillation under Reduced Pressure STEAM DISTILLATION Steam distillation is a special type of distillation used for temperature sensitive materials like natural aromatic compounds which are insoluble in water…

Introduction: Using solid-liquid extraction the fat trimyristin was successfully isolated from nutmeg. Using a chemical reaction called hydrolysis the isolated trimyristin was converted to myristic acid. Background: Solid-liquid extractions are common in everyday life and are important in a chemistry laboratory because they involve the separation of an individual compound from mixtures. Using solid-liquid extraction, which separates compounds based on solubility. Trimyristin was isolated from…

Introduction Extraction involves the separation of compounds in a mixture. This separation in based on the differences in each compound’s solubility. For the purpose of this lab, the liquid-liquid extraction technique was used. This method involves using two immiscible solvents, and because of each compounds solubility in each of those solvents, the mixed compounds can separate. Generally, this process includes water as one of the immiscible solvents, but for this lab, t-butyl methyl ether was…

A mixture of two unknown liquids were obtained. The assigned mixture code was recorded in the notebook (#10). The mixture of the two miscible liquids contained a low boiling point and high boiling point. The method used to separate the two miscible liquids was simple distillation and therefore a simple distillation apparatus was set up. The apparatus included 250mL distillation flask, a 100ml receiving flask, a condenser for water to run through, and a thermometer to monitor the temperature. The…

Name Institution Course: Instructor Date of Submission Separation (Liquid-Liquid Extraction) Lab Summary The aim of this lab was to separate a mixture of aniline, benzoic acid, and 1,4-dimethoxybenzene by the method of extraction. In this experiment, 7 ml of the mixture was used instead of 10 ml. A portion of this mixture was transferred to another flask to facilitate cooling. Afterwards, 3.5 ml of 12 M hydrochloric acid was added gradually to the mixture. A sample was obtained at a melting…

Hypothesis: If I were to extract DNA without proper chemicals and equipment, the result DNA would become faulty or poor quality for further investigation My hypothesis predicts that if we were to use improper or non-lab chemicals, the result DNA would become faulty and would result in an inadequate samples for further studies, it would seem that if we were to extract DNA without chemicals like lysis solution, concentrated salt solution, resuspenstion buffer, ethanol or isopropyl alcohol, it…

Results: Table 1: Raw data that shows how changing the mass(g) of soap in the extraction solution affects the mass of DNA extracted from the kiwi (g) Mass of DNA extracted from kiwi (g) Set Mass of Soap in extraction solution (g) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 1 0.5 1.25 0.74 0.24 2.04 0.30 2 1 1.41 1.38 1.26 1.52 1.36 3 1.5 1.91 1.48 2.39 2.63 2.00 4 2 2.43 3.34 3.26 3.01 3.62 5 2.5 2.65 4.00 5.32 3.52 3.95 All results are recorded with 2 decimal points for higher accuracy Numbers…

To carry out the experiment we need to get caffeine into a solution. We used a solid-liquid extraction by adding 14 tea bags into a 1L beaker with 6g of sodium carbonate, 250 mL of tap water and bringing this to a boil for five minutes on a hot plate. Sodium carbonate is a weak bronsted base, that neutralizes tannic acids extracted from tea, that interfere with the later sublimation operation.(1) We turned the heat off after five minutes, added ice cubes to cool the mixture to room temperature…

β-glucuronidase from Helix pomatia were added into 1mL of urine or serum sample. The enzymatic deconjugation was conducted in a water bath at 37 °C overnight. Then liquid-liquid extraction was performed on a Visiprep™ DL SPE vacuum manifold to isolate analytes from urine or serum. Chem Elute 1mL cartridge was used in the liquid-liquid extraction. The injection volume was 20 µL. A Thermo LTQ mass spectrometer was interfaced to a Finnigan Surveyor Autosampler…