Lane

3. Results 3.1 Cloning and phylogenetic analysis B. bigemina Argentina strain was used to obtain genomic and cDNA for PCR with primers BbigAMA-1 F and R to amplify the AMA-1 gene. The sequences had an identical length from both templates and of the estimated size according to primers' design (not shown). The nucleotide sequencing analysis presented a 1988-bp long gene (gene bank accession number: AB481200) and encoded a 595-amino acid peptide (gene bank accession number: BAH22706) (Fig. 1)…

Growing up, my dad taught me many things. He taught me how to ride my bike, tie my shoes, how to set a table and so much more. Out of all the lessons I’ve learned there is one thing that always stays with me: Don’t drive when you’re upset. Coming from a family with three older siblings, all four of us were taught this same rule. When my dad taught me this lesson, I was learning to drive at the age of seventeen. At the time, I understood the lesson but I never truly grasped it all until August 30…

pre game was the better moment out of this high school experience. During pre game the coaches told us to meet them in the meeting room at 4:00pm. When I walked into the meeting room it was silent and stillness in the cold room. I can remember coach Lane walking into the room silently and with the bible in his right hand. As we went around the room letting every player talk it finally came around to me. I stood up and turned my attention…

Baton Rouge road ways seem to lack a shoulder which I can kind of see why the traffic easily get backed up, there’s no lane to pull over to maybe change a flat of even service in issue on your vehicle. In fact, one day stuck in traffic I realized on the Mississippi Bridge there were no shoulder for cars who may need to access a shoulder for emergency situations. Acquiring…

students to put in more work both in high school and college. Knowing that money is at cost pushes students to maintain good grades and actually graduate. Working hard also pays off for students as tuition costs are lowered by grants and scholarships. (Lane). From the perspective of colleges, if they were government funded they wouldn’t have nearly as much money, and it’s possible that the quality of education being offered could drop. Incentives to provide the best education would be replaced…

revelation that occurs one London season between two friends, Jack Worthing and Algernon Moncrieff, and their paramours Gwendolyn Bracknell and Cecily Cardew respectively. Minor characters include Lady Bracknell, Gwendolen’s mother and aunt to Algernon; Lane, Algernon’s butler; Miss Prism, Cecily’s governess; Merriman, Cecily’s butler; and Dr. Chasuble, a reverend. The performance of the play that will be discussed in this essay was performed by TCNJ All College Theatre in 2015 . The play uses…

allowing what service or internet domain can be allowed through these “fast lanes” -or the normal speed the consumer subscribed to- that only the major internet service providers can chose or if the website owner 's pay to be choosed. And if the consumer wants to access more at a normal speed, they’ll have to pay a good premium to see the internet as it was intended (Betters, "What Is Net Neutrality and Internet Fast Lanes, and Why Is Obama Stepping in Now?"). Though the carriers claim that…

The game of basketball, starting with James Naismith, has become an immense part of American culture throughout the years. Many people throughout America enjoy the squeaks of basketball shoes cutting on the court, the sight of a newly waxed floor so shiny that one can see their reflection off of it, and the feel of the leathery bumps of the basketball beneath their fingers. This love of the game has caused much growth of basketball in the past years. There are many different levels of Basketball…

in the air like the fireflies that danced in and out of my vision. Pounding against the decrepit boards, my bare feet were getting tired from the running I was doing, but I couldn 't stop now. John was waiting for me near the ocean at the end of the lane, our little spot. Nothing more than a puny fishing hole, our "ocean" was a special muse no one knew about except me and John. Wandering home after school one day, I was fourteen and he was fifteen when we first found it. Through the years,…

limit, the construction of biking lanes, the safety education in the universities, and even the state laws. Indeed, private vehicle owners are prone to be more careful when driving on campus because more traffic signs are set inside the campus together with the 25mph speed limitation, preventing them from driving too fast or threatening the bicyclists by sharing the path. In addition, biking infrastructures at the universities such as the network of biking lanes and paths is improving in recent…