Lagging indicator

To determine optimal pH value for the hydrolysis of p-NPG by leukocyte GCase, we examined effects of variable pH (4.0-5.5) at a constant substrate concentration (5mM). Figure 1 shows the pH activity curves of leukocyte GCase assayed in the presence and absence of 6 mg per ml Sodium taurocholate. In the absence of the pure sodium taurocholate, the activity in the range of pH 4.0-4.8 was considerably lower. However, in the presence of pure Sodium taurocholate, leukocyte GCase was optimally active…

Experiment 3 titled “Physiologically Important IONS” and the purpose of this lab is to become familiar with metal and nonmetal ions and mostly important in biological and physiological processes. Also, to become familiar with tests to determine the presence or absence of ions as well as to estimate approximate amount. To begin, when a solution is tested, make sure you observe everything and note everything. This is lab, we will test some positive ion and determines if it’s an anion or a cation.…

The list of errors start from the beginning of the experiment. Although the numbers seem to be close to the actual values, there is still room for error with the numbers. What the class noticed first is that we could have been working with very impure benzoic acid. The benzoic acid we were given was made by the 102 chemistry students, meaning that it wasn’t properly made. Students have said that they were rushed and weren’t able to take their time to filter the solid out of the solution. We had…

Introduction Although, in general, salts are known for their solubility, not all types of salts completely dissociate in water. Some of them partially dissociates and creates a dynamic equilibrium due to the instances when their ions recombine to form the original salts. An example of this is potassium hydrogen tartrate (KHC4H4O6 or KHT). When KHT is placed in water, it partially dissolves and forms K+ and HC4H4O6- ions. This reaction is shown below: KHC_4 H_4 O_6 (s)⇄ K^+ (aq)+HC_4 H_4 O_6^-…

III. Results Table 2: KHP Vs. NaOH Titration data Trial Initial KHP acid Volume (ml) End KHP acid volume (ml) Volume difference (ml) Volume of NaOH Base Used (ml) 1 7 mL 16 mL 9 mL 10.0 mL 2 16 mL 27.5 mL 11.5 mL 10.0 mL 3 27.5 mL 38.5 mL 11 mL 10.0 mL Table 3: Titration of HCl with NaOH Trial Initial NaOH Volume (ml) End NaOH base volume (ml) Volume difference (ml) Volume of HCl acid Used (ml) 1 4.5 mL 14.5 mL 10 mL 10.0 mL 2 30 mL 41.5 mL 11.5 mL 10.0 mL 3 19 mL 31 mL 12 mL 10.0…

3.7.1. Effect of solvent and pH The influence of the solvent on Schiff base ligand L and its metal(II) complexes were studied. The results showed that the optimal solvent for Schiff base ligand L, Co(II), Ni(II) and Cu(II) complexes were CH3CN at λemi =320, 310, 316 and 330 nm, while the optimal solvent for Mn(II) complex was DMF λemi = 312 nm, as shown in Figure 7. The effect pH on the fluorescence intensity of Schiff base ligand L and its metal(II) complexes were investigated between…

Question 1: pH can be described as a measure of acidity or basicity (Alkalinity) of a solution. It is exactly equal in amount to the negative log of the concentration of hydronium(H3O+) ions. Due to the negative log relationship, increased hydronium ions transform to lower pH. When we add it to water, an acid releases hydrogen ion. Hydrogen ions in water unite with the water molecules to form hydronium ions, thereby lowering the pH and increasing the acidity of water. Water exposed to air is…

2. Materials and Methods: 2.1. Materials: Natural red, propidium iodide (PI), acridine orange and phosphate-buffered saline (PBS) were obtained from Sigma-Aldrich (St.Louis, MO). May–Grunwald–Giemsa stain was procured from Merck (Darmstadt, Germany). Fetal calf serum, Dulbecco's Modified Eagle Medium (DEMEM) and RPMI 1640 were bought from GIBCO/Life Technologies Inc. (Gaithersburg, MD). 2.2. Isolation and Proliferation of MSCs Bone marrow derived MSCs were isolated as described previously…

Project 3 Identification and Synthesis of an Unknown Ionic Compound Mitchell Morgan CEM-161-007 Josh Ward 28th October 2015 Abstract The chief objective of this experiment was to identify the unknown compound. The following tests were conducted to eventually establish the identity of the compounded: qualitative solubility tests, anion and cation tests, flame test, and the formation of a precipitate. The physical properties of the unknown ionic compound found that it has no smell and was…

Observations: This experiment was divided into two parts: one part was to observe the properties of a single Alka Seltzer tablet and the second part was to find the percentage of CO2. In the first part of the experiment, the visible properties of Alka Seltzer were observed and recorded. Each Alka Seltzer contains the same three ingredients: 325 mg of Aspirin, 1000 mg of Citric Acid, and 1916 mg of Sodium Bicarbonate. Two of Alka Seltzer’s ingredients, Citric Acid and Sodium Bicarbonate (Baking…