Uranium and its decay products can be found in the environment through a wide array of anthropogenic activities such as mining, nuclear and power generation industries and the production of phosphate fertilizers .(Sprynskyy et al. 2011) They pose a severe, long term ecological and public health threat (Khani 2011, Landa 2003) and therefore there is a considerable effort in the removal of these pollutants before their final discharge in the environment, as well as their recovery as an energy…
PRE FORMULATION STUDY: Pre formulation testing was an investigation of physical and chemical properties of a drug substance alone and when combined with excipients. It was the first step in the rational development of dosage forms. Class: -Pre formulation study can divide into two subclasses: API characterization, Compatibility study Active pharmaceutical ingredient (API) characterization:- Organoleptic evaluation:-These are preliminary characteristics of any substance which is useful in…
In this lab we wanted to identify what the four mystery liquids were using density and their characteristic properties. We were initially given four liquids that were labelled numbers 1- 4, some graduated cylinders, and a scale, but we also knew that the 4 liquids were ethanol, vegetable oil, water, and cyclohexane. Using the mass that we found when we measured the liquid and using only 10 mL of the liquids we were able to calculate the density and easily identify the liquids and see if they…
an acid or a base? There are different methods to discover whether a substance is an acid or a base. One of the methods is by using an acid-base indicator or by identifying hydrogen or hydroxide ions in a substance’s chemical formula. Certain materials such as a plastic template, red litmus paper, blue litmus paper, phenolphthalein, universal indicator, and a pH chart can be used to help us distinguish acids and bases. Before beginning this experiment, a lab coat and goggles should be put on…
Interpretation and Evaluation: Rinsing Process: When performing a volumetric analysis all equipment to be used must be washed with deionised water. This is done in order to remove anything that contaminate your solution and have an effect on the results. It is important that deionised water is used and not regular tap water, this is because tap water could itself contains many unwanted impurities such salts, other solids and bacteria. If these were to contaminate the solutions to be used it…
Malachite Green Assay: The reaction that AGPase catalyzes produces PPi. The resulting PPi that is produced is hydrolyzed by an inorganic pyrophosphatase (Sigma Aldrich) to generate phosphate (Pi). Each reaction for the assay was conducted on a 96 well plate and the reaction mixture was 50µL and contained Pre-Mix which contained 50mM HEPPS, pH=8.0 (MP Biomedicals), 10mM MgCl2, 1.5mM FBP (Sigma Aldrich), and 0.2mg/mL Bovine Serum Albumin (Sigma Aldrich), and 1.5mU/µL yeast inorganic…
were used to help understand the different behaviors of different concentrations. Household items were used to help apply basic theories about acids and bases such as pH. The titration curves were evaluated to help determine which indicator needed to be used for the indicator titration. Anions are ions that have more electrons than protons, therefore,…
The four methods used for quantification of alkalinity (4) are summarized below: •Use of a pH indicator: a change in the color from pure yellow to orange is observed and recorded when a known pH is reached. In this his experiment we used methyl orange as indicator The consumed volume until specific pH allow us to know how much volume is required to reach the endpoint. •Visual identification from titration curve; the data are recorded…
Water Quality Water is essential to human life and the health of the environment. Good water quality is essential for irrigation, drinking and recreation and also sustains ecological processes that support native fish populations, vegetation and bird life. We expect that our water supply will not only be safe, but will taste good, and look crystal clear. Water quality is commonly defined by its physical, chemical, biological and aesthetic (appearance and smell) characteristics. Water quality…
Natural Indicators Lab; a Study in pH Levels Using Green Cabbage Introduction- pH is the concentration of hydrogen in a substance. It is the scale used to tell how acidic or basic a solution is. The normal pH scale goes zero to fourteen. Zero to about six indicates that the solution is an acid. If the solution is closer to zero it makes it a stronger acid, but if the solution is closer to seven than it is a weak acid. If a solution has a pH of seven then it is neutral. A pH of eight to fourteen…