Lactose

is important in food products. Through this lab, we will get to grow Salmonella from contaminated sample and perform a series of test to confirm its presence. In this lab, Salmonella need to be enriched in a lactose broth first…

Hypothesis: Aerobic Respiration: If a phosphate buffer, dichlorophenol-indophenol and succinate are added to a mitochondrial extract then the succinate molecules will bind to an enzyme complex (succinate dehydrogenase). Because the enzyme and FAD are a part of the same complex, initiating succinate oxidation, this reaction reduces FAD to FADH2. Thus the oxidation of succinate to furmarate. Anaerobic Respiration: If a solution of yeast and glucose is combined and placed in different incubation…

took place in Italy. Two groups of people were gathered. One group with lactose intolerance was assembled and the other without lactose intolerance also were assembled. Both were given a "lactose" pill to consume. What they didn't know was that it was really a sugar pill. After the groups consumed there pills ,the case study showed that forty-four percent of those with lactose intolerance and twenty-six percent without lactose intolerance developed symptoms of gastrointestinal discomfort. So…

disease is a blood test for gluten antibodies, or a biopsy of the small bowel to assess if any damage has been done to the gut. Skin biopsies might also be taken. Lactose intolerance is caused by the body not producing enough lactase. Lactase is an enzyme secreted in the small intestine that digests substances containing lactose. If lactose is consumed, symptoms like stomach bloating,…

Scientists use different types of nutrient agar media to help with the study of microorganisms. The purpose of this experiment was to examine the different growths of minority organisms within a variety of media cultures. These cultures included types of selective, differential, and enriched media and were applied to split sections on the agar plate. Each of the four broth cultures: Escherichia coli, Enterobacter aerogenes, Staphylococcus epidermidis, and Streptococcus faecalis showed observable…

Violet red bile agar is a selective medium used to identify lactose-fermenting coliforms. This medium is typically used to test dairy products and water. The medium contains lactose which acts as a carbohydrate source, bile salts and crystal violet that act as an inhibitory agent towards gram-positive microorganisms and neutral red is added to serve as a pH indicator, microorganisms lower the pH which results in the colonies turning red. Some of the organisms that grow on this medium are:…

rapidly on agar media (O’Rear et al., 1992). Of the three sugar fermentation tests available a lactose test has been performed to determine if the unknown is capable of using lactose as its carbon source. The results obtained, no color change or gas bubbles, indicate the unknown specimen is not a lactose fermenter, so it is not using lactose as its source of carbon (a color change would indicate a lactose fermenter). The test confirms only S.marcescens and successfully eliminates the other two…

have to drink is something you do not like? If this has happened to you, you know how annoying it is. Right now at Foley Public schools the only option they have for a drink at lunch is milk. Milk may be healthy but kids who do not like milk and lactose intolerant kids are upset with the lack of drink options. When you go through the lunch line you are offered a fruit or starch, a vegetable and a milk. Most kids will take a milk but throw it away wasting most of the money spent on milk. Adding…

Introduction Life could not exist without microorganisms: plants depend on microbes to obtain soil and cows need microorganisms to digest cellulose. Microbes degrade waste and they live in our bodies to keep us healthy. Microorganisms are also used to make vaccines and antibiotics. However, some bacteria are known for causing diseases such as the common cold, AIDS, Herpes, and Chicken Pox. Knowing the identity of microbes is essential for knowing the causative agent of a disease in order to…

Using Appendix E of the General Microbiology Laboratory lab handbook, I narrowed down the unknown microorganism to Enterobacter aerogenes. Every test matched with my experimental results except for the fermentation of lactose. According to Appendix E, E.aerogenes is able to ferment lactose however my enterotube experiment yielded a negative result. I assume this difference is due to human error of misidentification of the color of the enterotube chamber. Besides, the MacConkey plate yielded a…