Lactose

meaning that it has a resistance to high salt environment and live in environments that are high in salt, but had a negative fermentation result meaning that it was unable to ferment mannitol. Also, this bacteria was had negative results for the lactose fermentation test meaning that the bacteria was unable to ferment bacteria, which produced no gas and also because there was no color change it means the bacteria could not produce…

There were many tests performed on my unknown, which led me to conclude that the unknown microorganism is Pseudomonas fluorescens. When determining the gram reaction of the unknown, a Gram stain was done. This process differentiates between a Gram-positive and Gram-negative microorganism. This is done in four steps. The microorganism was stained with iodine, crystal violet, alcohol and acetone, and safranin. Iodine was used first as it acts as a mordant. It forms an insoluble complex by binding…

popularity of milk is beginning to decline. According to the USDA, Americans drink 37% less milk on average today than they did in 1970 due to health concerns and the rise in substitutes. Alternative milk products such as almond, soy, and rice milk are lactose-free…

test indicates a gram-negative bacteria and a negative string test indicates a gram-positive bacteria. Another method that can classify gram-positive or gram-negative is the presence of bacterial growth on different media. Eosin Methlyene Blue-lactose (EMB-lactose) is an example of a media that allows gram-negative bacteria to grow and…

identification. Gram stain tests were performed in order to identify which unknown is gram positive and gram negative. Using selective and differential media, like MacConkey agar which allows the growth of gram negative bacteria only that are able to ferment lactose. Also, mannitol agar was used which isolates and detects gram-positive bacteria. As well as blood agar, which is a nutritive media with differential properties in respect to hemolysis. The catalase test indicated catalase production…

Identification of bacteria can be a difficult process due to the fact that individual bacterial cells can possess similar structural morphology to other types of bacteria. As we have demonstrated in previous lab experiments there are key biological and chemical processes that are characteristic to different types of bacteria. Using various biochemical tests we can evaluate and detect these different processes to aid in our identification of unknown bacterial samples. The implications of these…

The structure and function of carbohydrates (25 Marks) Carbohydrates are made from Carbon (C), Hydrogen (H) and Oxygen (O). A monosaccharide is the monomer sub unit of carbohydrates and can be joined together through a condensation reaction to form a glycosidic bond between the two monomers which is known as a disaccharide. The above diagram shows a condensation reaction taking place. There is a reaction between two monomer sub units, in this case α-glucose and ethanol, which involves the…

Introduction In this lab report I use two different techniques to identify Unknown A and Unknown B bacteria’s. These techniques are gram staining and metabolic testing. I first used Gram staining to distinguished and identify the bacteria’s. Han Christian discovered gram staining in 1882, he had biopsy a patient lung that had pneumonia. The main purpose for Gram staining is to identify if is Gram positive or Gram negative by using differential staining. While Gram staining shows the…

Are humans still evolving or have they stopped, has been a question for the modern world for a long time now. Evolution is one of the most researched topic which gives us an insight of the development of the human race throughout millions of years. In this research paper I will use peer reviewed articles to answer the question about if we are still evolving as humans. I will try to highlight the reasons that I think that humans are still evolving despite the advances in technology, medication,…

contain enzymes. Lactase is an enzyme that a lot of people know about. It cuts lactose into its smaller sugars: galactose and glucose. When the small intestine of a human does not produce a sufficient amount of this enzyme, this condition is known as the lactose intolerant, which is when a human has discomfort in the form of cramps, gas and diarrhea when they eat/drink milk products. Lactase is also used to make lactose-free products for people with this condition. Many other enzymes are present…