Klebsiella pneumoniae

An unknown sample #18 was obtained on November 6, 2017. The sample came in a glass test tube with a cotton swab that contain the unknown sample. To find the identity of the unknown sample, we had to conduct several biochemical tests to eliminate and narrow down possible organisms that fit the biochemical description to accurately identify our unknown organism of interest. These biochemical tests provided insight on the physical color retention of our unknown sample, and through’s the unknown…

The bacteria used in the experiment are Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, and Salmonella pneumonia. These all bacteria will be culture in the nutrient broth. The first stage is making Nutrient Broth. Weigh out 5.8 grams of nutrient broth powder. Dissolve completely the nutrient broth powder in beaker containing 200 ml of distilled or the deionise water. Then the mixture is transfers to the vials. Those vials are autoclave and sterilise at 1…

Explanation of a microbiological algorithm for Hospital-acquired and Ventilator associated pneumonia. This algorithm is intended to deal with the microbiological aspects of hospital-acquired and ventilator-associated pneumonia. The severity of the illness is important in determining when to collect the diagnostic samples and which antimicrobials to administer due to which organisms are present. There are many prognostic scoring systems used to suggest the severity of the disease in patients…

With this test, another test to figure out the “oxygen requirements” of the organism was done in a “thioglycollate agar tube” from which will allow the organism to grow either towards the top of the agar where oxygen exists, an “aerobic” microbe, or to the bottom of the tube where its considered “anaerobic”, or dispersed between the tube to show that it’s a “facultative anaerobe” (MB 352 Laboratory manual pg 47). Considering that after incubation, the organism did not grow through the tube but…

and legislative to raise funds in order to increase antibiotic production. The “ESKAPE” pathogens that are currently causing the majority of infections and are resistant to current antibiotics are Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter…

Discussion Microscope is an optical instrument that have magnifying lens which is used for inspecting objects that is too small to be seen or to be seen distinctly and in details by the unaided eye. Microscope also is an optical instrument that keep observe specimen in resolution and magnitude way. However, there are some microbes such as viruses that cannot be observe by certain microscope and this microbes has to be observe using electronic microscope which has more magnification power. In…

The 20th century saw a series of remarkable discoveries that changed the face of medical practice. Among the most important was the discovery of antimicrobial agents, beginning with the synthesis of arsphenamine by Paul Ehrlich as the century dawned [1]. With this discovery, the dreaded scourge of syphilis was brought under control, although not eradicated. However, the toxicity of the drug made it less than ideal as an antimicrobial agent. Shortly thereafter, optochin (ethyl cupreine) was tried…

Introduction/Review of Literature Introduction Hospital acquired infections (HAI), also known as healthcare associated infections or nosocomial infections, are infections transmitted to patients while in a hospital or other healthcare facility. According to the Centers for Disease Control and Prevention (2015), there were approximately 722,000 patients suffering from hospital acquired infections in the United States in 2011 of whom 75,000 suffered fatalities. Accordingly, hospital acquired…

lyophilized way in the microbiology laboratory of Fasa University of Medical Sciences: Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 29212, S.saprophyticus ATCC 15305, Enterococcus fecalis ATCC 29212, Proteus vulgaris PTCC 1079, Klebsiella pneumoniae ATCC 10031, Escherichia coli ATCC 25922. The leaves of C. viminalis were collected from the decorated trees in the city of Fasa in April 2015. The plant identity was confirmed in Department of Botany, Darab Payame Noor…

Abstract: Objective: To evaluate the ability of triple Taqman probe real-time PCR to the detection and differentiation of vanA, vanB and vanM genotypes of Vancomycin-resistant enterococci (VRE). METHODS: Choosing the culture method and PCR-sequencing assay as the reference standard，respectively, a total of 254 VRE and 90 strains of no-VRE were conducted by triple Taqman probe real-time PCR collected from 2005 to 2015 in China. Results: When detect the VRE strains, the sensitivity, specificity,…