Ion

reaction were tried for derivatization of pregabalin before GC-MS analysis. Derivatization was achived using N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA). Identification and quantification of a new derivative were performed in selected ion monitoring mode using electron impact ionization. Pregabalin was extracted from plasma by protein precipitation with acetonitrile. The extraction recovery ranged from 60.23-73.19 %. The GC-MS method was validated and the validation…

sequences. In addition to using these three different localizations to categorize receptors, the types of action of receptors are also used as a mean of classification. The four main classifications of receptors are: 1. Ionotropic (or ligand-gated ion channel) receptors, 2. G-protein coupled receptors (GPCR), 3. Enzyme or kinase linked receptors and 4. Nuclear receptors (not in terms of localization but effect on gene expression). Receptors are ligand-specific due to the unique structures of…

changes colours depending on the quantity of hydrogen ions in the solution. The red cabbage can produce red, purple or blue juice. The colour it produces depends on the pH of the water it has been mixed with.…

Introduction: As the world population increases continually, limited amounts of food sources will become a more pressing matter. Agriculture will become more important as a result of the increase in population. The quality of soil is of paramount importance when it comes to planting crops. A major factor attributed to soil quality is the presence of earthworms. Earthworms are very important in the world of agriculture and can help maximize crop yields. In this study, the effects of earthworms…

Calpain has been shown as a very prevalent protein found in a variety of organisms. There are a variety of different calpain proteins that have been discovered, each serving a different function [4]. Recent studies have indicated a correlation between the amount of calpain present and the loss of visual function due to various ocular degenerative diseases [2]. This study will be focusing on the adverse effects of calpain on the retina of mice, and how they differ from the norm. The eye is one of…

QUESTION Explain the significance and optimum value of the following parameter i. pH ii. DO iii. TDS iv. COD v. BOD ANSWER pH ph is the concentration of hydronium ion. It represents the ratio of hydronium ions (H3O + ) to Hydroxide ion (OH- ). It is numeric scale which decides the basic and alkalinity of water. If the ph of the solution is less than 7 than then the solution is said to alkaline and if the ph is greater then 7 then the solution is said to be basic. The ph of pure water at 25o C is…

recently in America. More than 2,400 roadsters have been sold in 31 countries as of 2008. It costs $109,000 and to many governments around the world this car is considered very capable and encouraged. It also happens to be the first car to use lithium-ion battery and the first electric car to go over 320 km per charge. In a champion ship called Federation Internationale de l’Automobile that involved a three day and 1,000 kilometer challenge, one of the formula one drivers drove a Tesla Roadster…

any effects on the body and they are removed from the body (Edta, 2016). So this means that adding EDTA has removed all the metal ions that were free in solution. As the specific activity of the Arginase is zero when EDTA is added it shows that Arginase required metal ions in solution to be active. This means that Arginase is a metallo-enzyme as it requires a metal ion to be…

it dissolved into solution as ions. A brown gas was observed forming in the beaker simultaneously as HNO3 is reduced and transformed to NO2, corresponding with the formation of nitrogen dioxide gas as the reaction progressed. The hydrated copper turned the water blue as the reaction was allowed to continue for about five minutes. Step 2: precipitation When sodium hydroxide (strong base) was reacted with copper(II) nitrate, the copper combined with the hydroxide ion to form an insoluble compound,…

purification steps, we must isolate the enzyme to be able to conclude it can be used for antibiotic use. Using homogenate, acid precipitation, ion exchange chromatography, and 2 from of gel filtration, the purification will be tested. I hypothesize these purification steps will isolate and purify the enzyme desired. Results Figure 1 shows Sephadex carboxymethyl ion exchange chromatography of a leukocyte’s cytoplasm. The 3 things being measured in this graph are: overall protein…