Interstellar medium

For this experiment, unknown sample #12 was used. The first step in identifying any unknown bacterium is to grow a culture on a nutrient agar plate. This bacterium was also plated on a MAC plate, which is both selective for gram-negative bacteria and differential as well. On the NA plate, bright red colonies were present; on the MAC plate there was bacterial growth as well, but no color change was observed. This lack of color change on the MAC plate indicated that the bacteria did not ferment…

Mtb is classified as a Gram-positive bacteria, and is one of the few bacteria that can be cultured in the laboratory5. Its cell surface is partly composed of various virulence lipids, such as phthiocerol dimycocerosates (PDIMs) and phenolic glycolipids (PGLs). and mycolic acid3. Mtb, like many other bacteria, is capable of sensing and responding to their surrounding environment. It does so via the use of two-component systems, one of which is called PhoPR two-component system6. In order for Mtb…

We first needed to collect all the proper materials to conduct the tests on our unknown bacterial sample. To begin the lab, we had to collect a nonpathogenic bacterial sample by swabbing an item or surface inside the biology building with a sterile cotton swab. Our lab group decided to swab a Sharpie permanent marker left at our lab station inside our lab room. We then opened a petri dish and smeared the swab across the surface of the agar several times. Once the bacteria was on the agar, we…

Though Robert Zemeckis’ Back to the Future is extremely successful in being a fun, blockbuster film, it does a very job in how it crafts the relationships between specific characters. Though Marty McFly (Michael J. Fox) is facing a struggle to get back home, there’s another struggle occurring in the movie between his father, George (Crispin Glover), and town bully, Biff Tannen (Thomas F. Wilson). By tracing their acting and relative staging of these two in certain shots, a narrative between them…

paragallinarum reference strains (HP1, HP 90 and HP144) and two Australian Av. paragallinarum reference strains (HP14 and HP60) were selected for growth curve construction. Three broths were tested for their suitability of growth, Haemophilus Test Medium Broth (TMB), Cation-adjusted Mueller-Hinton Broth (CAMHB) with supplements and Veterinary Fastidious Media (VFM). TMB was prepared with 1% Biosate Peptone (BD), 1% sodium chloride (Merck Millipore, Australia), 0.1% starch (Merck Millipore), 0.1%…

Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…

INT: Hello, I’m here with Lucy Brown who has just viewed, The Shawshank Redemption for the first time, and is willing to answer a few questions. Firstly, what qualities of Red and Andy’s characters do you believe brought them together? Brooke: Fantastic question. I believe that Red and Andy’s friendship developed majorly throughout their time in prison. Both Red and Andy started out as complete social opposites, Red from a poor background and Andy from a privileged white background. They both…

Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…

Objective I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient…

(the tool): A wire made into a loop to assist in picking up inoculum from a culture of microorganisms. The loop is also used in the process of cultivating microbes by transferring inoculum for streaking. 3. Agar vs Blood Agar: a. Agar: a growth medium made with agar and other nutrients. The agar allows microorganisms such as fungi and bacteria to be cultured. b. Blood Agar: Blood agar is used for the purpose of cultivation and isolation of various strains of pathogenic bacteria. 4.…